PHYSICAL MAP OF CHROMOSOME 5
PHYSICAL MAP OF CHROMOSOME 5
批准号:
2209076
负责人:
JOHN J WASMUTH
金额:
$129.73万
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-02-01 至 1996-07-31
中文摘要
该提案的长期目标是开发一个高
分辨率,人类 5 号染色体的连续物理图。该图将
使用几种不同但互补的方法构建,
包括:自然删除映射,它将细分
染色体分为 30 个独立的区室;辐射混合测绘
将提供整个染色体的有序且连续的图谱
至少 500 Kb 级别的分辨率;原位多色荧光
杂交,当辐射时将提供顺序信息
混合映射不能,最后,建立许多
酵母人工产物形式的克隆 DNA 的重叠片段
染色体 (YAC) 和粘粒重叠群,每个重叠群至少跨越
不同地区的 200 万个碱基对。 提出的一种方法是
实现后一个目标是一种利用辐射混合的新颖方法
从流式排序的 5 号染色体中开发区域特异性子文库
科斯米德图书馆。
物理地图上至少会放置 500 个标记,包括
至少100个高度多态性遗传标记和超过100个已知基因
功能。 每个标记将采用寡核苷酸引物的形式
用于 PCR(序列标记位点,或 STS)。 放置的遗传标记
物理地图将有助于将物理地图与
减数分裂或连锁图。 将两种地图关联起来可以提供
立即提取重要的生物和临床信息的强大方法
来自染色体的信息。 特别是,整合遗传和
物理图谱对于将图谱与疾病表型联系起来至关重要
与至少七种遗传性疾病有关,其基因
已知位于 5 号染色体上。此外,了解
已知功能的基因在物理图上的精确位置可以
对于评估他们作为参与的候选人来说是无价的
遗传性或获得性疾病,包括各种类型的白血病。
英文摘要
The long term objective of this proposal is to develop a high
resolution, contiguous physical map of human chromosome 5. The map will
be constructed using several different but complementary approaches,
including: natural deletion mapping, which will subdivide the
chromosome into 30 separate compartments; radiation hybrid mapping which
will provide an ordered and contiguous map of the entire chromosome of
at least a 500 Kb level of resolution; multicolor fluorescence in situ
hybridization, which will provide order information when radiation
hybrid mapping cannot and, finally, the establishment of many
overlapping segments of cloned DNA in the form of yeast artificial
chromosome (YAC) and cosmid contigs, each of which will span at least
two million base pairs in different regions. One method proposed to
achieve this latter goal is a novel approach using radiation hybrids to
develop region-specific sublibraries from a flow-sorted chromosome 5
cosmid library.
AT least 500 markers will be placed on the physical map, including at
least 100 highly polymorphic genetic markers and over 100 genes of known
function. Each marker will be in the form of oligonucleotide primers
for PCR (sequence tagged sites, or STSs). The genetic markers placed on
the physical map will serve to integrate the physical map with the
meiotic or linkage map. Relating the two kinds of maps provides a
powerful way to immediately extract important biological and clinical
information from the chromosome. In particular, integrating genetic and
physical maps is critical for relating the maps to disease phenotypes
associated with at least seven inherited disorders, the genes for which
are known to be on chromosome 5. In addition, a knowledge of the
precise location of genes of known function on the physical map can be
invaluable for assessing them as candidates for being involved for
inherited or acquired disorders, including various types of leukemia.
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会议论文
ISOLATION OF DISEASE GENES FROM CHROMOSOME 5
-
批准号:2081577
-
项目类别:
-
资助金额:$17.42万
-
财政年份:1994
-
负责人:JOHN J WASMUTH
-
依托单位:
INTERNATIONAL WORKSHOP ON HUMAN CHROMOSOME 5
-
批准号:3435547
-
项目类别:
-
资助金额:$1.78万
-
财政年份:1993
-
负责人:JOHN J WASMUTH
-
依托单位:
PHYSICAL MAP OF CHROMOSOME 5
-
批准号:2209075
-
项目类别:
-
资助金额:$134.91万
-
财政年份:1993
-
负责人:JOHN J WASMUTH
-
依托单位:
PHYSICAL MAP OF CHROMOSOME 5
-
批准号:3097346
-
项目类别:
-
资助金额:$120.6万
-
财政年份:1993
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:2265610
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:2265611
-
项目类别:
-
资助金额:$27.56万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:3410941
-
项目类别:
-
资助金额:$23.83万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:2265609
-
项目类别:
-
资助金额:$25.63万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTONS DISEASE GENE
-
批准号:3410942
-
项目类别:
-
资助金额:$24.65万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:3410938
-
项目类别:
-
资助金额:$24.14万
-
财政年份:1991
-
负责人:JOHN J WASMUTH
-
依托单位:
HIGH RESOLUTION PHYSICAL MAPS OF 4P AND 5Q
-
批准号:3333414
-
项目类别:
-
资助金额:$34.24万
-
财政年份:1989
-
负责人:JOHN J WASMUTH
-
依托单位:
HIGH RESOLUTION PHYSICAL MAPS OF 4P AND 5Q
-
批准号:3333415
-
项目类别:
-
资助金额:$44.37万
-
财政年份:1989
-
负责人:JOHN J WASMUTH
-
依托单位:
HIGH RESOLUTION PHYSICAL MAPS OF 4P AND 5Q
-
批准号:3333413
-
项目类别:
-
资助金额:$36.99万
-
财政年份:1989
-
负责人:JOHN J WASMUTH
-
依托单位:
HIGH RESOLUTION PHYSICAL MAPS OF 4P AND 5Q
-
批准号:3300849
-
项目类别:
-
资助金额:$36.11万
-
财政年份:1989
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:3410940
-
项目类别:
-
资助金额:$15.13万
-
财政年份:1988
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:3410937
-
项目类别:
-
资助金额:$15.56万
-
财政年份:1988
-
负责人:JOHN J WASMUTH
-
依托单位:
ISOLATION OF THE HUNTINGTON'S DISEASE GENE
-
批准号:3410939
-
项目类别:
-
资助金额:$15.03万
-
财政年份:1988
-
负责人:JOHN J WASMUTH
-
依托单位:
MOLECULAR GENETIC STUDY OF THE CRI DU CHAT SYNDROME
-
批准号:3315744
-
项目类别:
-
资助金额:$14.87万
-
财政年份:1984
-
负责人:JOHN J WASMUTH
-
依托单位:
MOLECULAR GENETIC STUDY OF THE CRI DU CHAT SYNDROME
-
批准号:3315745
-
项目类别:
-
资助金额:$15.02万
-
财政年份:1984
-
负责人:JOHN J WASMUTH
-
依托单位:
MOLECULAR GENETIC STUDY OF THE CRI DU CHAT SYNDROME
-
批准号:3315741
-
项目类别:
-
资助金额:$13.81万
-
财政年份:1984
-
负责人:JOHN J WASMUTH
-
依托单位: