PHYSICAL MAP OF CHROMOSOME 5

5 号染色体的物理图谱

• 批准号: 2209075
• 负责人: JOHN J WASMUTH
• 金额: $ 134.91万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2209075
• 关键词: genetic disorder; genetic library; genetic mapping; genetic markers; human genetic material tag

The long term objective of this proposal is to develop a high resolution, contiguous physical map of human chromosome 5. The map will be constructed using several different but complementary approaches, including: natural deletion mapping, which will subdivide the chromosome into 30 separate compartments; radiation hybrid mapping which will provide an ordered and contiguous map of the entire chromosome of at least a 500 Kb level of resolution; multicolor fluorescence in situ hybridization, which will provide order information when radiation hybrid mapping cannot and, finally, the establishment of many overlapping segments of cloned DNA in the form of yeast artificial chromosome (YAC) and cosmid contigs, each of which will span at least two million base pairs in different regions. One method proposed to achieve this latter goal is a novel approach using radiation hybrids to develop region-specific sublibraries from a flow-sorted chromosome 5 cosmid library. AT least 500 markers will be placed on the physical map, including at least 100 highly polymorphic genetic markers and over 100 genes of known function. Each marker will be in the form of oligonucleotide primers for PCR (sequence tagged sites, or STSs). The genetic markers placed on the physical map will serve to integrate the physical map with the meiotic or linkage map. Relating the two kinds of maps provides a powerful way to immediately extract important biological and clinical information from the chromosome. In particular, integrating genetic and physical maps is critical for relating the maps to disease phenotypes associated with at least seven inherited disorders, the genes for which are known to be on chromosome 5. In addition, a knowledge of the precise location of genes of known function on the physical map can be invaluable for assessing them as candidates for being involved for inherited or acquired disorders, including various types of leukemia.

这项建议的长远目标是发展一个高水平的 分辨率，人类5号染色体的连续物理图谱。 地图会 使用几种不同但互补的方法来构建， 包括：自然删除映射，它将细分 染色体分成30个独立的隔室;辐射杂交作图， 将提供一个有序的和连续的整个染色体的地图， 至少500 Kb的分辨率水平;原位荧光 杂交，当辐射时将提供顺序信息， 混合映射不能，最后，建立许多 酵母人工合成形式的克隆DNA的重叠片段 染色体（YAC）和粘粒重叠群，其中每一个将跨越至少 两百万个碱基对分布在不同的区域 提出的一种方法， 实现后一个目标是一种使用辐射混合器的新方法， 从流式分选的5号染色体开发区域特异性亚文库 粘粒文库。 至少有500个标记将被放置在物理地图上，包括在 至少100个高度多态性的遗传标记和超过100个已知的 功能 每个标记物将以寡核苷酸引物的形式 PCR（序列标记位点，或STS）。 基因标记放在 物理地图将用于将物理地图与 减数分裂或连锁图谱。 将这两种地图联系起来， 立即提取重要的生物和临床 来自染色体的信息。 特别是，整合遗传和 物理图谱对于将图谱与疾病表型相关联至关重要 与至少七种遗传性疾病有关， 都在5号染色体上 此外， 在物理图谱上已知功能的基因的精确位置可以 对于评估他们作为参与的候选人是非常宝贵的， 遗传性或获得性疾病，包括各种类型的白血病。