CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
基本信息
- 批准号:2183725
- 负责人:
- 金额:$ 24.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-07-01 至 1999-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The long-term objectives of this project are to identify and characterize
the forces that underlie chromosome movement in meiosis and mitosis,
including interactions of chromosomes with the spindle and microtubule
dynamics involved in spindle assembly and function.
The proposed studies focus on ncd, a Drosophila protein related to the
microtubule motor protein, kinesin. Ncd is a minus-end microtubule motor
required for normal chromosome segregation in oocyte meiosis and mitosis
in early embryos. The ncd protein is associated with meiotic spindles and
mitotic spindle fibers and centrosomes.
The proposed studies will test the hypotheses that 1) ncd functions to
assemble bipolar spindles in meiosis and move chromosomes poleward in
meiosis and mitosis, and 2) interactions of ncd with microtubules during
its mechanochemical cycle differ from those of kinesin, and account for
the differences in motility properties of ncd and kinesin.
Specific aims are to:
1. Visualize spindle assembly and chromosome movement in wildtype and
mutant oocytes and embryos using transformants carrying the A. Victoria
green fluorescent protein (GFP) fused to ncd.
2. Screen for and analyze new modifier mutants of ncd to identify proteins
that interact with specific ncd mutant alleles.
3. Determine the tubulin subunit(s) to which ncd binds and the site(s) of
binding using genetic interactions of ncd with tubulin mutants, molecular
analysis, and in vitro binding studies.
4. Design, construct and analyze mutants that specifically affect the ncd
ATPase in order to obtain information regarding interactions of the ncd
motor with microtubules.
The proposed studies address the basis of chromosome movement during
meiosis and mitosis, and the role and mechanism of function of the ncd
microtubule motor protein. Results of these studies will provide
information regarding the molecular forces needed for proper chromosome
distribution in dividing cells. Defective chromosome distribution causes
aneuploidy and genetic abnormalities, and is associated with cellular
transformation in humans.
这个项目的长期目标是识别和描述
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Sharyn A. Endow其他文献
Two restriction-like enzymes from <em>Xanthomonas malvacearum</em>
- DOI:
10.1016/s0022-2836(77)80198-8 - 发表时间:
1977-05-25 - 期刊:
- 影响因子:
- 作者:
Sharyn A. Endow;Richard J. Roberts - 通讯作者:
Richard J. Roberts
Determinants of molecular motor directionality
分子马达方向性的决定因素
- DOI:
10.1038/14113 - 发表时间:
1999-10-01 - 期刊:
- 影响因子:19.100
- 作者:
Sharyn A. Endow - 通讯作者:
Sharyn A. Endow
A new crystal structure of a kinesin mutant with greater mechanical output than wild type—identification of a structural element involved in force production
- DOI:
10.1016/j.bpj.2021.11.760 - 发表时间:
2022-02-11 - 期刊:
- 影响因子:
- 作者:
Matthew Y. Wang;Yuanyuan Wei;Jobichen Chacko;Jayaraman Sivaraman;Sharyn A. Endow - 通讯作者:
Sharyn A. Endow
Microtubule Binding and Rotation of the Kinesin-14 Stalk
- DOI:
10.1016/j.bpj.2008.12.2624 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Sharyn A. Endow;Zhang-Yi Liang;Mark A. Hallen - 通讯作者:
Mark A. Hallen
Structural Analysis of a Human Mitotic Kinesin and Its Potential Binding Site for a Small Molecule Inhibitor
- DOI:
10.1016/j.bpj.2017.11.1087 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Hee-Won Park;Zhujun Ma;Haizhong Zhu;Shimin Jiang;Robert C. Robinson;Sharyn A. Endow - 通讯作者:
Sharyn A. Endow
Sharyn A. Endow的其他文献
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{{ truncateString('Sharyn A. Endow', 18)}}的其他基金
Kinesin Force Production and Biomechanics of Division
驱动蛋白力的产生和分裂的生物力学
- 批准号:
10452616 - 财政年份:2021
- 资助金额:
$ 24.47万 - 项目类别:
Kinesin Force Production and Biomechanics of Division
驱动蛋白力的产生和分裂的生物力学
- 批准号:
10302986 - 财政年份:2021
- 资助金额:
$ 24.47万 - 项目类别: