CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS

减数分裂和有丝分裂中的染色体运动

基本信息

  • 批准号:
    2183725
  • 负责人:
  • 金额:
    $ 24.47万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1991
  • 资助国家:
    美国
  • 起止时间:
    1991-07-01 至 1999-06-30
  • 项目状态:
    已结题

项目摘要

The long-term objectives of this project are to identify and characterize the forces that underlie chromosome movement in meiosis and mitosis, including interactions of chromosomes with the spindle and microtubule dynamics involved in spindle assembly and function. The proposed studies focus on ncd, a Drosophila protein related to the microtubule motor protein, kinesin. Ncd is a minus-end microtubule motor required for normal chromosome segregation in oocyte meiosis and mitosis in early embryos. The ncd protein is associated with meiotic spindles and mitotic spindle fibers and centrosomes. The proposed studies will test the hypotheses that 1) ncd functions to assemble bipolar spindles in meiosis and move chromosomes poleward in meiosis and mitosis, and 2) interactions of ncd with microtubules during its mechanochemical cycle differ from those of kinesin, and account for the differences in motility properties of ncd and kinesin. Specific aims are to: 1. Visualize spindle assembly and chromosome movement in wildtype and mutant oocytes and embryos using transformants carrying the A. Victoria green fluorescent protein (GFP) fused to ncd. 2. Screen for and analyze new modifier mutants of ncd to identify proteins that interact with specific ncd mutant alleles. 3. Determine the tubulin subunit(s) to which ncd binds and the site(s) of binding using genetic interactions of ncd with tubulin mutants, molecular analysis, and in vitro binding studies. 4. Design, construct and analyze mutants that specifically affect the ncd ATPase in order to obtain information regarding interactions of the ncd motor with microtubules. The proposed studies address the basis of chromosome movement during meiosis and mitosis, and the role and mechanism of function of the ncd microtubule motor protein. Results of these studies will provide information regarding the molecular forces needed for proper chromosome distribution in dividing cells. Defective chromosome distribution causes aneuploidy and genetic abnormalities, and is associated with cellular transformation in humans.
这个项目的长期目标是识别和描述

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Sharyn A. Endow其他文献

Two restriction-like enzymes from <em>Xanthomonas malvacearum</em>
  • DOI:
    10.1016/s0022-2836(77)80198-8
  • 发表时间:
    1977-05-25
  • 期刊:
  • 影响因子:
  • 作者:
    Sharyn A. Endow;Richard J. Roberts
  • 通讯作者:
    Richard J. Roberts
Determinants of molecular motor directionality
分子马达方向性的决定因素
  • DOI:
    10.1038/14113
  • 发表时间:
    1999-10-01
  • 期刊:
  • 影响因子:
    19.100
  • 作者:
    Sharyn A. Endow
  • 通讯作者:
    Sharyn A. Endow
A new crystal structure of a kinesin mutant with greater mechanical output than wild type—identification of a structural element involved in force production
  • DOI:
    10.1016/j.bpj.2021.11.760
  • 发表时间:
    2022-02-11
  • 期刊:
  • 影响因子:
  • 作者:
    Matthew Y. Wang;Yuanyuan Wei;Jobichen Chacko;Jayaraman Sivaraman;Sharyn A. Endow
  • 通讯作者:
    Sharyn A. Endow
Microtubule Binding and Rotation of the Kinesin-14 Stalk
  • DOI:
    10.1016/j.bpj.2008.12.2624
  • 发表时间:
    2009-02-01
  • 期刊:
  • 影响因子:
  • 作者:
    Sharyn A. Endow;Zhang-Yi Liang;Mark A. Hallen
  • 通讯作者:
    Mark A. Hallen
A First-Approximation Estimate of Forces Required for Microtubule Breakage
  • DOI:
    10.1016/j.bpj.2018.11.1396
  • 发表时间:
    2019-02-15
  • 期刊:
  • 影响因子:
  • 作者:
    Sharyn A. Endow;Piotr E. Marszalek
  • 通讯作者:
    Piotr E. Marszalek

Sharyn A. Endow的其他文献

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{{ truncateString('Sharyn A. Endow', 18)}}的其他基金

Kinesin Force Production and Biomechanics of Division
驱动蛋白力的产生和分裂的生物力学
  • 批准号:
    10452616
  • 财政年份:
    2021
  • 资助金额:
    $ 24.47万
  • 项目类别:
Kinesin Force Production and Biomechanics of Division
驱动蛋白力的产生和分裂的生物力学
  • 批准号:
    10302986
  • 财政年份:
    2021
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7931526
  • 财政年份:
    2009
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    6342846
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    6627171
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    3305612
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    3305613
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7632207
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    2183724
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7872868
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:

相似海外基金

Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7931526
  • 财政年份:
    2009
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    6342846
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    6627171
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    3305612
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    3305613
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7632207
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
CHROMOSOME MOVEMENT IN MEIOSIS AND MITOSIS
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    2183724
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7193383
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7872868
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
Chromosome Movement in Meiosis and Mitosis
减数分裂和有丝分裂中的染色体运动
  • 批准号:
    7367341
  • 财政年份:
    1991
  • 资助金额:
    $ 24.47万
  • 项目类别:
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