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PRODUCT-MEDIATED REGULATION OF ORNITHINE DECARBOXYLASE

PRODUCT-MEDIATED REGULATION OF ORNITHINE DECARBOXYLASE
产品介导的鸟氨酸脱羧酶调节
批准号：
2183089
负责人：
Philip Coffino
金额：
$16.31万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
项目状态：
已结题
起止时间：
1991-01-01 至 1994-12-31
来源：
https://reporter.nih.gov/project-details/2183089
关键词：
SDS polyacrylamide gel electrophoresis Trypanosoma affinity chromatography biological information processing complementary DNA conformation crosslink gene expression genetic regulation genetic translation genetically modified animals immunoprecipitation laboratory mouse messenger RNA molecular cloning ornithine decarboxylase polyamines polymerase chain reaction posttranslational modifications protein biosynthesis protein folding protein purification protein sequence protein signal sequence protein structure recombinant DNA site directed mutagenesis southern blotting transfection

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英文摘要
Mammalian ornithine decarboxylase (ODC) is subject to a form of feedback regulation whereby polyamines, the product of the metabolic pathway initiated by ODC, cause a prompt reduction of enzymatic activity when their level becomes high within cells. This process occurs post- translationally, but its mechanism is not well understood. ODC of the parasite Trypanosome brucei is structurally similar to mammalian ODC, but is not regulated in this way. Utilizing cloned genes from the parasite and from mouse, we will employ reverse-genetic and biochemical approaches to studying this problem. In addition, transgenic mice will be used to determine whether the regulatory process mediates significant biological functions in the intact organism.
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Structure of functionally important dynamic states of the proteasome
  • 批准号：
    9130874
  • 项目类别：
  • 资助金额：
    $44.28万
  • 财政年份：
    2014
  • 负责人：
    Philip Coffino
  • 依托单位：
    ROCKEFELLER UNIVERSITY
Structure of functionally important dynamic states of the proteasome
  • 批准号：
    8925908
  • 项目类别：
  • 资助金额：
    $46.02万
  • 财政年份：
    2014
  • 负责人：
    Philip Coffino
  • 依托单位：
    ROCKEFELLER UNIVERSITY
Structure of functionally important dynamic states of the proteasome
Structure of functionally important dynamic states of the proteasome
  • 批准号：
    9339698
  • 项目类别：
  • 资助金额：
    $44.28万
  • 财政年份：
    2014
  • 负责人：
    Philip Coffino
  • 依托单位：
    ROCKEFELLER UNIVERSITY
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