PRODUCT MEDIATED REGULATION OF ORNITHINE DECARBOXYLASE

产品介导的鸟氨酸脱羧酶调节

• 批准号: 3304779
• 负责人: Philip Coffino
• 金额: $ 14.82万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304779
• 关键词: SDS polyacrylamide gel electrophoresis; Trypanosoma; affinity chromatography; biological information processing; complementary DNA; conformation; crosslink; gene expression; genetic regulation; genetic translation; genetically modified animals; immunoprecipitation; laboratory mouse; messenger RNA; molecular cloning; ornithine decarboxylase; polyamines; polymerase chain reaction; posttranslational modifications; protein biosynthesis; protein folding; protein purification; protein sequence; protein signal sequence; protein structure; recombinant DNA; site directed mutagenesis; southern blotting; transfection

Mammalian ornithine decarboxylase (ODC) is subject to a form of feedback regulation whereby polyamines, the product of the metabolic pathway initiated by ODC, cause a prompt reduction of enzymatic activity when their level becomes high within cells. This process occurs post- translationally, but its mechanism is not well understood. ODC of the parasite Trypanosome brucei is structurally similar to mammalian ODC, but is not regulated in this way. Utilizing cloned genes from the parasite and from mouse, we will employ reverse-genetic and biochemical approaches to studying this problem. In addition, transgenic mice will be used to determine whether the regulatory process mediates significant biological functions in the intact organism.

哺乳动物鸟氨酸脱羧酶（ODC）受到一种反馈形式 调节多胺，代谢途径的乘积 由ODC发起的，当酶活性迅速降低 水平在细胞内变高。这个过程是在翻译后发生的， 但是它的机制尚未得到充分理解。寄生虫锥虫的ODC Brucei在结构上与哺乳动物ODC相似，但不受调节 这边走。利用寄生虫和小鼠的克隆基因，我们将 采用反遗传和生化方法来研究这个问题。 此外，转基因小鼠将用于确定是否是否 调节过程介导完整的重要生物学功能 生物。