A novel biochemical nanomotor

描述(申请人提供)：纳米电机将化学或其他能源转化为运动，施加可以移动外部货物的力。它们是纳米技术的重要组成部分。这个项目的总体目标是开发一种可以沿着线性聚合物移动的生化纳米马达。这样的装置将被用作动力纳米装置的模块。它将受到控制和指挥，以便沿着指定的轨道将货物运送到特定的地址。为此，我们将使用依赖于ATP的蛋白质ClpX，它参与、展开和移动蛋白质。在其天然的细菌环境中，ClpX的功能是将蛋白质底物输送到蛋白酶中。我们将对其进行改造，使其沿着DNA移动，从而使用ClpX作为ATP供电的绞车，以DNA为缆线。为了实现这些目标，我们将进行以下实验。具体目标1：构建包含(1)与ClpX结合并启动易位的蛋白质或肽的嵌合对象，该嵌合对象与(2)ClpX随后继续沿其移动的DNA相连。具体目标2：开发和应用评估ClpX沿DNA动力易位的方法。具体目标3：使用这些分析方法，修改DNA的性质，以优化转位速度和功率。利用来自不同细菌种类的大肠杆菌ClpX和ClpX的诱变来改善马达所需的特性。 与公共健康相关：物体叙事纳米摩托将化学或其他能源转化为运动，施加可以移动外部货物的力量。它们是纳米技术的重要组成部分。我们将改造细菌蛋白ClpX，它参与、展开和移动蛋白质，开发它作为一种新型的生化纳米马达，沿着DNA移动。

DESCRIPTION (provided by applicant): Nanomotors convert chemical or other energy sources into motion, exerting forces that can move an external cargo. They are an important part of nanotechnology. The overall goal of this project is to develop a biochemical nanomotor that moves along a linear polymer. Such a device will be used as a module within a powered nanodevice. It will be controlled and directed so as to deliver cargo along specified tracks to specific addresses. To that end we will use the ATP-dependent protein ClpX, which engages, unfolds and moves proteins. In its native bacterial context ClpX functions to feed protein substrates into a protease. We will adapt it to move instead along DNA, thereby using ClpX as an ATP-powered winch with DNA as a cable. In furtherance of these goals we will perform the following experiments. Specific Aim 1: Construct chimeric objects consisting of (1) a protein or peptide that engages ClpX and initiates translocation, linked to (2) a DNA along which ClpX subsequently continues to move. Specific Aim 2: Develop and apply assays to assess the powered translocation of ClpX along DNA. Specific Aim 3: Using these assays, modify the properties of DNA to optimize translocation velocity and power. Use mutagenesis of E. coli ClpX and ClpX sourced from diverse bacterial species to improve the desired properties of the motor. PUBLIC HEALTH RELEVANCE: oject Narrative Nanomotors convert chemical or other energy sources into motion, exerting forces that can move an external cargo. They are an important part of nanotechnology. We will adapt the bacterial protein ClpX, which engages, unfolds and moves proteins, developing it to function as a novel biochemical nanomotor that moves along DNA.