A novel biochemical nanomotor

一种新型生化纳米马达

• 批准号: 7821413
• 负责人: Philip Coffino
• 金额: $ 19.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7821413
• 关键词: Address; Bacterial Proteins; Biochemical; Biological Assay; Chemicals; ClpX protein; DNA; Devices; Energy-Generating Resources; Escherichia coli; Exploratory/Developmental Grant; Goals; Link; Motion; Motor; Mutagenesis; Nanotechnology; Peptide Hydrolases; Peptides; Polymers; Property; Proteins; Specific qualifier value; feeding; improved; nanodevice; novel; public health relevance; research study

DESCRIPTION (provided by applicant): Nanomotors convert chemical or other energy sources into motion, exerting forces that can move an external cargo. They are an important part of nanotechnology. The overall goal of this project is to develop a biochemical nanomotor that moves along a linear polymer. Such a device will be used as a module within a powered nanodevice. It will be controlled and directed so as to deliver cargo along specified tracks to specific addresses. To that end we will use the ATP-dependent protein ClpX, which engages, unfolds and moves proteins. In its native bacterial context ClpX functions to feed protein substrates into a protease. We will adapt it to move instead along DNA, thereby using ClpX as an ATP-powered winch with DNA as a cable. In furtherance of these goals we will perform the following experiments. Specific Aim 1: Construct chimeric objects consisting of (1) a protein or peptide that engages ClpX and initiates translocation, linked to (2) a DNA along which ClpX subsequently continues to move. Specific Aim 2: Develop and apply assays to assess the powered translocation of ClpX along DNA. Specific Aim 3: Using these assays, modify the properties of DNA to optimize translocation velocity and power. Use mutagenesis of E. coli ClpX and ClpX sourced from diverse bacterial species to improve the desired properties of the motor. PUBLIC HEALTH RELEVANCE: oject Narrative Nanomotors convert chemical or other energy sources into motion, exerting forces that can move an external cargo. They are an important part of nanotechnology. We will adapt the bacterial protein ClpX, which engages, unfolds and moves proteins, developing it to function as a novel biochemical nanomotor that moves along DNA.

描述（由申请人提供）：纳米运动员将化学或其他能源转换为运动，施加可以移动外部货物的力。它们是纳米技术的重要组成部分。该项目的总体目标是开发沿线性聚合物移动的生化纳米能力。这样的设备将用作电源纳米电视中的模块。它将受到控制和指示，以便沿着指定的轨道运送货物到特定地址。为此，我们将使用依赖ATP的蛋白质CLPX，该蛋白CLPX参与，展开和移动蛋白质。在其天然细菌环境中，Clpx功能可将蛋白质底物喂入蛋白酶。我们将其调整以沿DNA移动，从而将CLPX用作ATP驱动的绞车，并用DNA作为电缆。为了促进这些目标，我们将执行以下实验。特定目标1：构造嵌合物体，由（1）蛋白质或肽组成，该蛋白质或肽与CLPX接合并启动易位，与（2）DNA链接到clpx随后继续移动的DNA。特定目标2：开发和应用测定法以评估CLPX沿DNA的动力易位。特定目标3：使用这些测定，修改DNA的特性以优化易位速度和功率。使用来自不同细菌物种的大肠杆菌CLPX和CLPX的诱变，以改善电动机的所需特性。 公共卫生相关性：注射叙事纳米运动员将化学或其他能源转化为运动，施加可以移动外部货物的力。它们是纳米技术的重要组成部分。我们将适应细菌蛋白CLPX，该蛋白CLPX参与，展开和移动蛋白质，从而将其发育为沿DNA移动的新型生化纳米动物。