HUMAN SMALL NUCLEAR RNAS WITH NOVEL NUCLEOTIDE SEQUENCES
具有新型核苷酸序列的人小核 RNA
基本信息
- 批准号:3303773
- 负责人:
- 金额:$ 17.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-05-01 至 1995-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have synthesized and Cloned novel cDNA probes for three new human small
nucleolar RNAs of unique nucleotide sequences, that will be named R, S and
T. RNAs R, S and T appear to be true small RNA species, as opposed to
breakdown products of large RNAs, because their cDNAs hybridize to a small
RNA, but not to large RNA, in agarose gel Northern blots. Our long-term
objective is to determine the function of RNAs R, S and T. It is
reasonable to suspect that these small RNAs are involved in some aspect of
ribosome production, because of their nucleolar location. Sequence
complementarities between RNAs R-T and pre-rRNA are compatible with
possible roles in pre-rRNA processing. This proposal focuses on testing
the hypothesis that these small RNAs may function in the pathway of
ribosome biogenesis. Our first goal is to determine the sequence of the
-42 nucleotides located at the 5' end of RNA S. Some molecules of RNAs T
and S are psoralen-crosslinked in vivo to large nucleolar RNA. This large
nucleolar RNA consists of two main bands when hybridized with the S DNA
probe: one migrates near 28S rRNA and the other is larger. Our second goal
is to find out whether a small percentage of the molecules of RNAs R-T: a)
behave as if specifically associated with pre-rRNA RNP particles or
pre-rRNA and b) can be specifically psoralen-crosslinked in vivo to
pre-rRNA, and, if so, to map approximately the location of the
intermolecular crosslink in pre-rRNA. The third goal is the identification
of the probes or system necessary to specifically lower the level of
available, intact molecules of RNAs R-T. One antisense
oligodeoxynucleotide targets the specific degradation of the RNA T present
in a nucleolar extract. The first choice is to locate the accessible
regions of RNAs R-T in nucleolar extracts and whole cells, using antisense
oligodeoxynucleotide-targeted degradation. Alternative approaches are
discussed. Nucleotide sequences that are highly conserved through
evolution tend to be functionally important. Then, in our fourth goal a
phylogenetic comparison of the nucleotide sequences of RNAs R-T should
identify the conserved sequences. The fifth goal is to test the effect of
the induced decrease of the level of available, intact molecules of RNAs
R, S and T on the pathway of ribosome formation in whole cells or cell-free
systems. There are important questions about the proteins with which these
small RNAs may associate, and about the 5' ends of these small RNAs, using
as probes currently available antibodies. We wish to ask those questions
as this project progresses, when time allows it.
我们合成并克隆了三个新的人类小分子的cdna探针。
唯一核苷酸序列的核仁RNA,将命名为R,S和
T.RNAs R、S和T似乎是真正的小RNA种,而不是
大RNA的分解产物,因为它们的cDNA杂交成小的
琼脂糖凝胶Northern blotts中的RNA,但不是大的RNA。我们的长期合作
目的:确定R、S和T三种核糖核酸的功能。
有理由怀疑这些小RNA参与了
核糖体的产生,因为它们的核仁位置。数列
RNA R-T和Pre-rRNA之间的互补性与
在前rRNA加工中可能扮演的角色。这份提案的重点是测试
假设这些小RNA可能在以下途径发挥作用
核糖体生物发生。我们的第一个目标是确定
-42个核苷酸位于RNAS的5‘端。一些RNAs T分子
和S在体内将补骨脂素交联到大核仁RNA上。这么大
核仁核糖核酸与S脱氧核糖核酸杂交时由两条主带组成
探针:一个在28S rRNA附近迁移,另一个较大。我们的第二个目标
是为了找出一小部分RNA分子R-T:A)
表现得就像是与前rRNA RNP颗粒或
Pre-rRNA和b)可以在体内特异性地与补骨脂素交联,从而
Pre-rRNA,如果是这样的话,映射大约
Pre-rRNA中的分子间交联。第三个目标是识别
所需的探头或系统来明确降低
可用的、完整的RNAs R-T分子一种反义
寡核苷酸靶向RNA T的特异性降解
在核仁提取液中。第一个选择是找到可访问的
利用反义技术研究核仁提取液和全细胞中RNAs R-T区域
寡核苷酸靶向降解。另一种方法是
讨论过。高度保守的核苷酸序列
进化往往在功能上很重要。然后,在我们的第四个目标a
RNAs R-T核苷酸序列的系统发育比较应
鉴定保守序列。第五个目标是测试
导致可利用的、完整的RNA分子水平的下降
R、S和T在全细胞和无细胞核糖体形成途径中的应用
系统。有一些关于蛋白质的重要问题,这些蛋白质
小RNA可以结合在这些小RNA的5‘端左右,使用
作为目前可用的抗体探针。我们想问这些问题
随着这个项目的进展,在时间允许的情况下。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
George L Eliceiri其他文献
George L Eliceiri的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('George L Eliceiri', 18)}}的其他基金
INTRACELLULAR LOCALIZATION OF SMALL NUCLEOLAR RNAS
小核仁 RNA 的细胞内定位
- 批准号:
2771098 - 财政年份:1997
- 资助金额:
$ 17.67万 - 项目类别:
INTRACELLULAR LOCALIZATION OF SMALL NUCLEOLAR RNAS
小核仁 RNA 的细胞内定位
- 批准号:
6019329 - 财政年份:1997
- 资助金额:
$ 17.67万 - 项目类别:
INTRACELLULAR LOCALIZATION OF SMALL NUCLEOLAR RNAS
小核仁 RNA 的细胞内定位
- 批准号:
2383441 - 财政年份:1997
- 资助金额:
$ 17.67万 - 项目类别:
HUMAN SMALL NUCLEAR RNAS WITH NOVEL NUCLEOTIDE SEQUENCES
具有新型核苷酸序列的人小核 RNA
- 批准号:
3303772 - 财政年份:1991
- 资助金额:
$ 17.67万 - 项目类别:
HUMAN SMALL NUCLEAR RNAS WITH NOVEL NUCLEOTIDE SEQUENCES
具有新型核苷酸序列的人小核 RNA
- 批准号:
3303771 - 财政年份:1991
- 资助金额:
$ 17.67万 - 项目类别:
相似海外基金
The European Xenopus Resource Centre
欧洲爪蟾资源中心
- 批准号:
BB/X018601/1 - 财政年份:2023
- 资助金额:
$ 17.67万 - 项目类别:
Research Grant
Localized mitochondrial metabolic activity in Xenopus mesendoderm cells undergoing collective cell migration
爪蟾中内胚层细胞集体细胞迁移的局部线粒体代谢活性
- 批准号:
10751722 - 财政年份:2023
- 资助金额:
$ 17.67万 - 项目类别:
Conference: 19th International Xenopus Conference
会议:第19届国际爪蟾会议
- 批准号:
2323251 - 财政年份:2023
- 资助金额:
$ 17.67万 - 项目类别:
Standard Grant
Analysis of Congenital Hydrocephalus Genes in Xenopus
非洲爪蟾先天性脑积水基因分析
- 批准号:
10626955 - 财政年份:2022
- 资助金额:
$ 17.67万 - 项目类别:
In vivo persistence and immuno-pathogenesis of Mycobacterium abscessus in a new Xenopus tadpole model
脓肿分枝杆菌在新爪蟾蝌蚪模型中的体内持久性和免疫发病机制
- 批准号:
10350750 - 财政年份:2022
- 资助金额:
$ 17.67万 - 项目类别:
Analysis of Congenital Hydrocephalus Genes in Xenopus
非洲爪蟾先天性脑积水基因分析
- 批准号:
10502642 - 财政年份:2022
- 资助金额:
$ 17.67万 - 项目类别:
Development and function of the Xenopus tadpole retinotegmental projection
非洲爪蟾蝌蚪视网膜被盖投射的发育和功能
- 批准号:
2212591 - 财政年份:2022
- 资助金额:
$ 17.67万 - 项目类别:
Standard Grant