REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
批准号:
2185155
负责人:
William A. Fonzi
金额:
$0.4万
依托单位国家:
美国
项目类别:
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-05-01 至 1994-09-30
关键词:
Candida albicans DNA footprinting biological polymorphism developmental genetics fungal genetics fusion gene gene deletion mutation gene expression genetic manipulation genetic promoter element genetic transcription histogenesis immunofluorescence technique molecular cloning mutant nucleic acid sequence southern blotting
中文摘要
用形态特异的杂交探针筛选一个cDNA.
文库中白色念珠菌在形成菌丝的过程中,顺序
以确定在形态发生过程中差异表达的基因。
至少21个不同的基因,在
鉴定并克隆了2个二态转换基因。使用转换
技术,我们将继续研究的重点是
控制二态反应过程中的基因表达。具体的
目的是研究这些基因在二态现象中的作用。
操纵它们的表达,构建空等位基因和
不适当表达的等位基因,并研究它们的调节
通过体内和体外启动子分析进行表达。
白念珠菌酵母-菌丝二型性的研究
令人信服的是,广泛的病原真菌展示了
菌丝形态在菌丝生长中的作用
白色念珠菌的致病机制。我们的防控试验
差异表达基因的转录旨在
理解二形性,而不是转录本身,并使用
一系列分子遗传技术现已可用。了解以下内容
负责基因差异表达的机制将是一个
我们对白色念珠菌如何反应的理解迈出了重要一步
它的环境,并调节二型性的反应。
英文摘要
Morphology-specific hybridization probes were used to screen a cDNA
library from Candida albicans in the process of forming hyphae, in order
to identify genes that are differentially expressed during morphogenesis.
At least 21 different genes, differentially expressed during the
dimorphic transition, were identified and cloned. Using transformation
techniques we will focus our continuing studies on the mechanisms for
controlling gene expression during the dimorphic response. The specific
aims are to study the role of these genes in dimorphism by genetic
manipulation of their expression, constructing null alleles and
inappropriately expressed alleles, and to study the regulation of their
expression by in vivo and in vitro promoter analysis.
Studies on yeast-hyphal dimorphism in Candida albicans are made
compelling by the wide range of pathogenic fungi that exhibit the
phenomenon and the possible role that hyphal forms play in the
pathogenesis of Candida albicans. Our experiments on the control of
transcription of differentially expressed genes are aimed at
understanding dimorphism, rather than transcription per se, and employ a
range of molecular genetic techniques now available. Knowledge of the
mechanisms responsible for the differential expression of gene will be a
significant step in our understanding of how Candida albicans responds to
its environment and regulates the dimorphic response.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
NICHE SPECIFIC PATHOBIOLOGY OF CANDIDA ALBICANS
-
批准号:6020060
-
项目类别:
-
资助金额:$21.86万
-
财政年份:1999
-
负责人:William A. Fonzi
-
依托单位:
NICHE SPECIFIC PATHOBIOLOGY OF CANDIDA ALBICANS
-
批准号:6532800
-
项目类别:
-
资助金额:$24.0万
-
财政年份:1999
-
负责人:William A. Fonzi
-
依托单位:
NICHE SPECIFIC PATHOBIOLOGY OF CANDIDA ALBICANS
-
批准号:6170654
-
项目类别:
-
资助金额:$22.63万
-
财政年份:1999
-
负责人:William A. Fonzi
-
依托单位:
NICHE SPECIFIC PATHOBIOLOGY OF CANDIDA ALBICANS
-
批准号:6374297
-
项目类别:
-
资助金额:$23.31万
-
财政年份:1999
-
负责人:William A. Fonzi
-
依托单位:
ENVIRONMENTAL MODULATION OF CANDIDAL SURFACE
-
批准号:6268190
-
项目类别:
-
资助金额:$11.24万
-
财政年份:1998
-
负责人:William A. Fonzi
-
依托单位:
ENVIRONMENTAL MODULATION OF CANDIDAL SURFACE
-
批准号:6235295
-
项目类别:
-
资助金额:$10.8万
-
财政年份:1997
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:2900784
-
项目类别:
-
资助金额:$24.22万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:6179414
-
项目类别:
-
资助金额:$24.95万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
ELONGATION FACTOR 3--TARGET FOR ANTICRYPTOCOCCAL DRUGS
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批准号:2066742
-
项目类别:
-
资助金额:$10.83万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:2685001
-
项目类别:
-
资助金额:$23.52万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
ELONGATION FACTOR-3: A TARGET FOR ANTICRYPTOCOCCAL DRUGS
-
批准号:3146802
-
项目类别:
-
资助金额:$17.63万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:3307159
-
项目类别:
-
资助金额:$19.63万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
Role of pH-regulated Genes of Candida Albicans
-
批准号:6408974
-
项目类别:
-
资助金额:$29.49万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
Role of pH-regulated Genes of Candida Albicans
-
批准号:6632600
-
项目类别:
-
资助金额:$31.04万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
Role of pH-regulated Genes of Candida Albicans
-
批准号:6741460
-
项目类别:
-
资助金额:$31.04万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:2185156
-
项目类别:
-
资助金额:$18.89万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
-
批准号:2022593
-
项目类别:
-
资助金额:$22.83万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
Role of pH-regulated Genes of Candida Albicans
-
批准号:6511819
-
项目类别:
-
资助金额:$31.04万
-
财政年份:1992
-
负责人:William A. Fonzi
-
依托单位:
POLYAMINE SYNTHESIS IN MICROBIAL CELLS
-
批准号:3295439
-
项目类别:
-
资助金额:$12.16万
-
财政年份:1988
-
负责人:William A. Fonzi
-
依托单位:
POLYAMINE SYNTHESIS IN MICROBIAL CELLS
-
批准号:3295440
-
项目类别:
-
资助金额:$12.65万
-
财政年份:1988
-
负责人:William A. Fonzi
-
依托单位:
海外基金