REGULATION OF DIMORPHISM IN CANDIDA ALBICANS

用形态特异的杂交探针筛选一个cDNA. 文库中白色念珠菌在形成菌丝的过程中，顺序 以确定在形态发生过程中差异表达的基因。 至少21个不同的基因，在 鉴定并克隆了2个二态转换基因。使用转换 技术，我们将继续研究的重点是 控制二态反应过程中的基因表达。具体的 目的是研究这些基因在二态现象中的作用。 操纵它们的表达，构建空等位基因和 不适当表达的等位基因，并研究它们的调节 通过体内和体外启动子分析进行表达。 白念珠菌酵母-菌丝二型性的研究 令人信服的是，广泛的病原真菌展示了 菌丝形态在菌丝生长中的作用 白色念珠菌的致病机制。我们的防控试验 差异表达基因的转录旨在 理解二形性，而不是转录本身，并使用 一系列分子遗传技术现已可用。了解以下内容 负责基因差异表达的机制将是一个 我们对白色念珠菌如何反应的理解迈出了重要一步 它的环境，并调节二型性的反应。

Morphology-specific hybridization probes were used to screen a cDNA library from Candida albicans in the process of forming hyphae, in order to identify genes that are differentially expressed during morphogenesis. At least 21 different genes, differentially expressed during the dimorphic transition, were identified and cloned. Using transformation techniques we will focus our continuing studies on the mechanisms for controlling gene expression during the dimorphic response. The specific aims are to study the role of these genes in dimorphism by genetic manipulation of their expression, constructing null alleles and inappropriately expressed alleles, and to study the regulation of their expression by in vivo and in vitro promoter analysis. Studies on yeast-hyphal dimorphism in Candida albicans are made compelling by the wide range of pathogenic fungi that exhibit the phenomenon and the possible role that hyphal forms play in the pathogenesis of Candida albicans. Our experiments on the control of transcription of differentially expressed genes are aimed at understanding dimorphism, rather than transcription per se, and employ a range of molecular genetic techniques now available. Knowledge of the mechanisms responsible for the differential expression of gene will be a significant step in our understanding of how Candida albicans responds to its environment and regulates the dimorphic response.