REGULATION OF DIMORPHISM IN CANDIDA ALBICANS

形态学特异性杂交探针用于筛选cDNA 从白色念珠菌在形成菌丝的过程中的文库中，按顺序 以鉴定在形态发生过程中差异表达的基因。 至少有21个不同的基因，差异表达的过程中 二态性转变，鉴定和克隆。 使用变换 技术，我们将集中我们继续研究的机制， 在二型反应期间控制基因表达。 具体 目的是通过遗传学方法研究这些基因在二态性中的作用。 操纵它们的表达，构建无效等位基因， 不适当表达的等位基因，并研究其调控 通过体内和体外启动子分析进行表达。 本文报道了白念珠菌酵母-菌丝二型性的研究 令人信服的是，广泛的致病真菌表现出 现象和可能的作用，菌丝形式发挥在 白色念珠菌的发病机制。 我们的实验控制了 差异表达基因的转录旨在 理解二态性，而不是转录本身，并采用 一系列分子遗传学技术。 知识 基因差异表达的机制将是一个 在我们理解白色念珠菌如何对 它的环境和调节二态反应。

Morphology-specific hybridization probes were used to screen a cDNA library from Candida albicans in the process of forming hyphae, in order to identify genes that are differentially expressed during morphogenesis. At least 21 different genes, differentially expressed during the dimorphic transition, were identified and cloned. Using transformation techniques we will focus our continuing studies on the mechanisms for controlling gene expression during the dimorphic response. The specific aims are to study the role of these genes in dimorphism by genetic manipulation of their expression, constructing null alleles and inappropriately expressed alleles, and to study the regulation of their expression by in vivo and in vitro promoter analysis. Studies on yeast-hyphal dimorphism in Candida albicans are made compelling by the wide range of pathogenic fungi that exhibit the phenomenon and the possible role that hyphal forms play in the pathogenesis of Candida albicans. Our experiments on the control of transcription of differentially expressed genes are aimed at understanding dimorphism, rather than transcription per se, and employ a range of molecular genetic techniques now available. Knowledge of the mechanisms responsible for the differential expression of gene will be a significant step in our understanding of how Candida albicans responds to its environment and regulates the dimorphic response.