REGULATION OF DIMORPHISM IN CANDIDA ALBICANS
白色念珠菌二态性的调控
基本信息
- 批准号:6179414
- 负责人:
- 金额:$ 24.95万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-05-01 至 2001-06-30
- 项目状态:已结题
- 来源:
- 关键词:Candida albicans acidity /alkalinity biological polymorphism cell wall developmental genetics enzyme activity fluorescence microscopy fungal genetics gene deletion mutation gene expression genetic regulation histogenesis hydrolase immunofluorescence technique molecular cloning mutant nucleic acid sequence polysaccharides reporter genes site directed mutagenesis transferase
项目摘要
Candida albicans is the most often cited agent of opportunitistic
fungal infections. The incidence of candidal infections have
increased substantially over the past decade and concerns over
this trend are heightened by the lack of efficacious treatments
and high mortality rates. Despite a voluminous literature on C.
Albicans, dealing largely with diagnostic techniques and research
into its pathogenicity, much of its biology remains hidden. The
mechanism(s) of pathogenesis continues to be a topic of
conjecture and debate, however, the dimorphic ability of C.
Albicans is regarded a significant contributing factor. The
investigator previously identified several candidal genes
differentially expressed in relation to the dimorphic response.
One of these genes, PHR1, is transcriptionally regulated in
responses to ambient pH, an environmental signal known to
modulate the dimorphic transition of C. Albicans. A second
pH-regulated gene, PHR2, encodes a structural and functional
homolog of PHR1. Genetic studies have demonstrated that both are
required for morphogenesis and virulence and probably encode an
essential cell function. Preliminary studies suggest that these
genes encode a glycosidase/glycosyl transferase activity that
participates in cell wall assembly. While this function is not
specific to hypase, the investigator has found a hyphal-specific
gene that is dependent upon PHR1 expression. This indicates that
the progression of morphogenesis is monitored by the cell and
that for some hyphal-specific genes, expression is directly
linked to morphogenesis. The investigator has proposed a series
of biochemical and genetic experiments to further define the
structure and function of the proteins encoded by PHR1 and PHR2
and to define the molecular mechanism(s) that integrate
morphogenesis with hyphal-specific gene expression. This
information will provide fundamental insights into the process
of candidal morphogenesis and will contribute to the
investigators long term objective of understanding the molecular
basis of dimorphism and its role in pathogenesis.
白色念珠菌是最常被引用的机会主义者的代理人
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Use of URA3 as a reporter of gene expression in C. albicans.
使用 URA3 作为白色念珠菌基因表达的报告基因。
- DOI:10.1007/bf00326156
- 发表时间:1995
- 期刊:
- 影响因子:2.5
- 作者:Myers,KK;Sypherd,PS;Fonzi,WA
- 通讯作者:Fonzi,WA
Spontaneous second-site suppressors of the filamentation defect of prr1Delta mutants define a critical domain of Rim101p in Candida albicans.
prr1Delta 突变体丝状化缺陷的自发第二位点抑制因子定义了白色念珠菌中 Rim101p 的关键结构域。
- DOI:10.1007/s004380100581
- 发表时间:2001
- 期刊:
- 影响因子:0
- 作者:Porta,A;Wang,Z;Ramon,A;Muhlschlegel,FA;Fonzi,WA
- 通讯作者:Fonzi,WA
Allele-specific gene targeting in Candida albicans results from heterology between alleles.
白色念珠菌中的等位基因特异性基因靶向源于等位基因之间的异源性。
- DOI:10.1099/00221287-146-9-2097
- 发表时间:2000
- 期刊:
- 影响因子:0
- 作者:Yesland,Kyle;Fonzi,WilliamA
- 通讯作者:Fonzi,WilliamA
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William A. Fonzi其他文献
Molecular responses to changes in the environmental pH are conserved between the fungal pathogens <em>Candida dubliniensis</em> and <em>Candida albicans</em>
- DOI:
10.1016/s1438-4221(00)80120-4 - 发表时间:
2000-07-01 - 期刊:
- 影响因子:
- 作者:
Werner J. Heinz;Oliver Kurzai;Axel A. Brakhage;William A. Fonzi;Hans-C. Korting;Matthias Frosch;Fritz A. Mühlschlegel - 通讯作者:
Fritz A. Mühlschlegel
The Fungal Colony, edited by N.A.R. Gow, G.D. Robson, and G.M. Gadd 1999.
- DOI:
10.1023/a:1007161221779 - 发表时间:
2001-02-01 - 期刊:
- 影响因子:2.900
- 作者:
William A. Fonzi - 通讯作者:
William A. Fonzi
William A. Fonzi的其他文献
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{{ truncateString('William A. Fonzi', 18)}}的其他基金
ELONGATION FACTOR 3--TARGET FOR ANTICRYPTOCOCCAL DRUGS
延伸因子 3——抗隐球菌药物的靶标
- 批准号:
2066742 - 财政年份:1992
- 资助金额:
$ 24.95万 - 项目类别: