ENVIRONMENTAL MODULATION OF CANDIDAL SURFACE

念珠菌表面的环境调节

• 批准号: 6235295
• 负责人: William A. Fonzi
• 金额: $ 10.8万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235295
• 关键词: Candida albicans; chimeric proteins; fusion gene; gene expression; gene mutation; genetic library; host organism interaction; microorganism culture; nucleic acid hybridization; nucleic acid sequence; protein structure function; reporter genes

With the long term objective of understanding the interactions between Candida albicans and its human host this proposal examines the structural and functional dynamics of the Candidal cell surface. The microbial cell surface as the interface between the microbe and its environment is responsible for mediating host/parasite interactions and pathogenesis. The cell surface of C. albicans is dynamic, exhibiting changes in morphology, adherence and other properties relevant to the pathogenic process. These changes occur in response to culture conditions and exemplify adaptive responses that likely occur in vivo. The molecular basis of cell surface dynamics is as yet poorly defined. Molecular genetics provides a means of defining the molecular basis of cell surface structure and dynamics and this application focuses on the analysis of genes encoding cell surface proteins which are differentially expressed in response to environmental conditions. Because the dynamic changes in the cell surface result in altered properties relevant to the host/parasite interaction and because these changes occur in response to culture conditions, it follows that the differentially expressed genes encode biochemical or structural functions that are directly involved in establishing cell surface characteristics integral to the pathogenic process. Analysis of the structure and function of these genes will provide information essential to our understanding of C. albicans and its interaction with the host.

长期目标是了解 白色念珠菌和它的人类宿主这个建议检查的结构 和细胞表面的功能动力学。 该微生物细胞 表面作为微生物与其环境之间的界面， 负责介导宿主/寄生虫相互作用和发病机制。 C.白色念珠菌是动态的， 形态、粘附性和与致病性相关的其他性质 过程 这些变化是对培养条件的反应， 可能发生在体内的适应性反应。 分子 细胞表面动力学的基础尚未明确。 分子遗传学提供了一种手段来定义的分子基础， 细胞表面结构和动力学，该应用程序侧重于 分析编码细胞表面蛋白质的基因，所述细胞表面蛋白质与细胞表面蛋白质的差异 以响应环境条件。 因为动态 细胞表面的变化导致与细胞相关的性质改变 宿主/寄生虫的相互作用，因为这些变化发生在响应 培养条件下，差异表达的基因 编码直接参与的生物化学或结构功能， 建立与病原体整合的细胞表面特征 过程 对这些基因的结构和功能的分析， 为我们理解C提供必要的信息。白色念珠菌及其 与宿主的互动。