FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG

干扰素诱导的泛素同系物的功能

基本信息

  • 批准号:
    2184840
  • 负责人:
  • 金额:
    $ 19.91万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1992
  • 资助国家:
    美国
  • 起止时间:
    1992-04-01 至 1999-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (adapted from the applicant's abstract): Cell line specific effects of the interferons are mediated through the coordinated induction of a subset of genes expressed at precise times during the cytokine response. The principal investigator has shown that p15, a 17 kDa early gene product of type 1 interferon induction, bears marked homology to a tandem di-ubiquitin sequence. Subsequent evidence suggests the biological response of this Ubiquitin Cross Reactive Protein (UCRP) is mediated through covalent ligation to a small subpopulation of intracellular target proteins. Recent results indicate UCRP conjugation to target proteins proceeds through a ligation pathway distinct from that of ubiquitin. This novel mechanism for interferon action will be examined in six specific aims. (1) Physical characterization of UCRP-- the stability of recombinant UCRP and its precursor to various structural perturbants will be monitored by CD and fluorescence quench for comparison to ubiquitin. (2) Examine the binding of UCRP to intermediate filaments--transient expression of UCRP-chloramphenicol aminotransferase (CAT) in cultured A549 cells will be used to confirm earlier immunohistochemical data suggesting UCRP serves as a trans acting binding determinant for noncovalent association of target proteins with intermediate filaments. Deletion analysis of UCRP-CAT constructs will be utilized to identify the binding motif on UCRP responsible for filament association. Partial microsequencing will be used to characterize three novel low molecular weight (15-17 kDa) intermediate filament-associated proteins found to bind UCRP. (3) Examine the dynamics of intracellular UCRP pools--direct assays and complementation studies of (125)1-UCRP conjugation in cultured A549 cell extracts will examine the regulation of the ligation reaction during interferon-beta induction. Potential roles for UCRP in the interferon response will be tested by blocking UCRP synthesis through transient expression of antisense UCRP MRNA. (4) Purify and characterize the preUCRP processing activity. (5) Examine the enzymology of UCRP conjugation--in vitro (125)1-UCRP conjugation assays will be employed to identify the enzymes(s) present in A549 extracts catalyzing polypeptide ligation. These enzymes will be purified and characterized for comparison to the ubiquitin conjugation pathway. (6) Cloning of the UCRP conjugating enzymes--partial microsequencing of the UCRP conjugating enzymes will be used to construct probes for screening an interferon-induced A549 cDNA library. Cloning and expression of the UCRP conjugating enzymes for subsequent mechanistic studies will provide functional comparisons to the parallel ubiquitin ligation pathway.
描述(改编自申请人摘要):细胞系特异性

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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ARTHUR L HAAS其他文献

ARTHUR L HAAS的其他文献

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{{ truncateString('ARTHUR L HAAS', 18)}}的其他基金

ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing
用于核酸测序的 ABI 3100 基因分析仪
  • 批准号:
    6578668
  • 财政年份:
    2003
  • 资助金额:
    $ 19.91万
  • 项目类别:
FASEB CONFERENCE ON UBIQUITIN AND PROTEIN DEGRADATION
FASEB 泛素和蛋白质降解会议
  • 批准号:
    2024156
  • 财政年份:
    1997
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    6519488
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON-INDUCED UBIQUITION HOMOLOG
干扰素诱导的泛在同系物的功能
  • 批准号:
    3306922
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    6202892
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    2184841
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    6386298
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    6920554
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    2392167
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
  • 批准号:
    2684995
  • 财政年份:
    1992
  • 资助金额:
    $ 19.91万
  • 项目类别:

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超生奥本海默化学中的化学结合理论及其在复杂分子系统中的应用
  • 批准号:
    20H00373
  • 财政年份:
    2020
  • 资助金额:
    $ 19.91万
  • 项目类别:
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