FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
基本信息
- 批准号:2184840
- 负责人:
- 金额:$ 19.91万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-04-01 至 1999-03-31
- 项目状态:已结题
- 来源:
- 关键词:antisense nucleic acid chemical binding chemical conjugate chemical stability circular dichroism covalent bond enzyme mechanism fluorescence gene induction /repression interferons intermediate filaments molecular cloning posttranslational modifications protein purification protein sequence protein structure function recombinant proteins tissue /cell culture ubiquitin
项目摘要
DESCRIPTION (adapted from the applicant's abstract): Cell line specific
effects of the interferons are mediated through the coordinated induction
of a subset of genes expressed at precise times during the cytokine
response. The principal investigator has shown that p15, a 17 kDa early
gene product of type 1 interferon induction, bears marked homology to
a tandem di-ubiquitin sequence. Subsequent evidence suggests the
biological response of this Ubiquitin Cross Reactive Protein (UCRP) is
mediated through covalent ligation to a small subpopulation of
intracellular target proteins. Recent results indicate UCRP conjugation
to target proteins proceeds through a ligation pathway distinct from
that of ubiquitin. This novel mechanism for interferon action will be
examined in six specific aims. (1) Physical characterization of UCRP--
the stability of recombinant UCRP and its precursor to various
structural perturbants will be monitored by CD and fluorescence quench
for comparison to ubiquitin. (2) Examine the binding of UCRP to
intermediate filaments--transient expression of UCRP-chloramphenicol
aminotransferase (CAT) in cultured A549 cells will be used to confirm
earlier immunohistochemical data suggesting UCRP serves as a trans
acting binding determinant for noncovalent association of target proteins
with intermediate filaments. Deletion analysis of UCRP-CAT constructs
will be utilized to identify the binding motif on UCRP responsible for
filament association. Partial microsequencing will be used to
characterize three novel low molecular weight (15-17 kDa) intermediate
filament-associated proteins found to bind UCRP. (3) Examine the
dynamics of intracellular UCRP pools--direct assays and complementation
studies of (125)1-UCRP conjugation in cultured A549 cell extracts will
examine the regulation of the ligation reaction during interferon-beta
induction. Potential roles for UCRP in the interferon response will be
tested by blocking UCRP synthesis through transient expression of
antisense UCRP MRNA. (4) Purify and characterize the preUCRP processing
activity. (5) Examine the enzymology of UCRP conjugation--in vitro
(125)1-UCRP conjugation assays will be employed to identify the
enzymes(s) present in A549 extracts catalyzing polypeptide ligation.
These enzymes will be purified and characterized for comparison to the
ubiquitin conjugation pathway. (6) Cloning of the UCRP conjugating
enzymes--partial microsequencing of the UCRP conjugating enzymes will
be used to construct probes for screening an interferon-induced A549
cDNA library. Cloning and expression of the UCRP conjugating enzymes
for subsequent mechanistic studies will provide functional comparisons
to the parallel ubiquitin ligation pathway.
描述(改编自申请人摘要):细胞系特异性
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ARTHUR L HAAS其他文献
ARTHUR L HAAS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ARTHUR L HAAS', 18)}}的其他基金
ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing
用于核酸测序的 ABI 3100 基因分析仪
- 批准号:
6578668 - 财政年份:2003
- 资助金额:
$ 19.91万 - 项目类别:
FASEB CONFERENCE ON UBIQUITIN AND PROTEIN DEGRADATION
FASEB 泛素和蛋白质降解会议
- 批准号:
2024156 - 财政年份:1997
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
6519488 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON-INDUCED UBIQUITION HOMOLOG
干扰素诱导的泛在同系物的功能
- 批准号:
3306922 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
6202892 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
2184841 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
6386298 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
6920554 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
2392167 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
干扰素诱导的泛素同系物的功能
- 批准号:
2684995 - 财政年份:1992
- 资助金额:
$ 19.91万 - 项目类别:
相似海外基金
Theory of chemical binding in beyond-Born-Oppenheimer chemistry and its applications to complex molecular systems
超生奥本海默化学中的化学结合理论及其在复杂分子系统中的应用
- 批准号:
20H00373 - 财政年份:2020
- 资助金额:
$ 19.91万 - 项目类别:
Grant-in-Aid for Scientific Research (A)