ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing

用于核酸测序的 ABI 3100 基因分析仪

• 批准号: 6578668
• 负责人: ARTHUR L HAAS
• 金额: $ 15.11万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6578668
• 关键词: biomedical equipment purchase; capillary electrophoresis; centrifugation; nucleic acid sequence; polymerase chain reaction

DESCRIPTION (provided by applicant): An Applied Biosystems ABI Prism 3100 Genetic Analyzer system is required for both ongoing and proposed studies requiring high throughput DNA sequencing by 24 investigators at the Medical College of Wisconsin. The proposed instrument will become part of the Institutional Protein and Nucleic Acid Core Facility of the Medical College, the oldest and most successful investigator-initiated institutional core facility available to all researchers without restriction. The ABI 3100 DNA sequencer will be sited in and operated by trained staff of the Core Facility. The 16 channels automated ABI 3100 instrument will replace an existing single channel ABI 310 DNA sequencer currently used by the Protein and Nucleic Acid Core Facility since 1999. Steadily increasing sample submissions due to markedly enhanced funding by and expansion in the number of Medical College investigators has exceeded the maximum capacity of the existing ABI 310 instrument. The proposed ABI 3100 DNA sequencer possesses a sample capacity that exceeds projected institutional needs for the foreseeable future yet retains the format and chemistry employed by the existing instrument and similar dedicated ABI 310 sequencers used by several Departments and groups, thus allowing seamless transitions for investigators using the latter instruments who choose to use the Protein and Nucleic Acid Core Facility. In addition, the proposed instrument will allow longer reads than currently available on the ABI 310 instrument.

描述（由申请人提供）：应用生物系统公司ABI Prism 3100基因分析仪系统是威斯康星医学院24名研究人员正在进行和拟议的高通量DNA测序研究所需的。拟议的仪器将成为医学院机构蛋白质和核酸核心设施的一部分，这是所有研究人员不受限制地使用的最古老和最成功的研究者发起的机构核心设施。ABI 3100 DNA测序仪将放置在核心设施内，并由训练有素的工作人员操作。16通道自动ABI 3100仪器将取代现有的单通道ABI 310 DNA测序仪，目前由蛋白质和核酸核心设施自1999年以来使用。由于医学院研究人员经费的显著增加和人数的增加，提交的样品稳步增加，超过了现有ABI 310仪器的最大容量。拟议的ABI 3100 DNA测序仪在可预见的未来拥有超过预计机构需求的样品容量，但保留了现有仪器和几个部门和小组使用的类似专用ABI 310测序仪所使用的格式和化学成分，从而允许使用后一种仪器选择使用蛋白质和核酸核心设施的研究人员无缝过渡。此外，拟议的仪器将允许比目前ABI 310仪器更长的读取时间。