ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing

描述（由申请人提供）：威斯康星州医学院的24名研究者正在进行的和拟定的需要高通量DNA测序的研究均需要使用Applied Biosystems ABI Prism 3100遗传分析仪系统。拟议的仪器将成为医学院的机构蛋白质和核酸核心设施的一部分，这是所有研究人员可以不受限制地使用的最古老和最成功的机构核心设施。ABI 3100 DNA测序仪将安装在核心设施内，并由经过培训的工作人员操作。16通道自动化ABI 3100仪器将取代自1999年以来蛋白质和核酸核心设施目前使用的现有单通道ABI 310 DNA测序仪。由于医学院研究人员的资助显著增加和数量的扩大，提交的样本稳步增加，已经超过了现有ABI 310仪器的最大容量。拟议的ABI 3100 DNA测序仪拥有的样本容量超过了可预见的未来的预计机构需求，但保留了现有仪器和几个部门和团体使用的类似专用ABI 310测序仪所采用的格式和化学，从而允许使用后一种仪器的研究人员无缝过渡，他们选择使用蛋白质和核酸核心设施。此外，拟议的仪器将允许比ABI 310仪器目前可用的更长的读数。

DESCRIPTION (provided by applicant): An Applied Biosystems ABI Prism 3100 Genetic Analyzer system is required for both ongoing and proposed studies requiring high throughput DNA sequencing by 24 investigators at the Medical College of Wisconsin. The proposed instrument will become part of the Institutional Protein and Nucleic Acid Core Facility of the Medical College, the oldest and most successful investigator-initiated institutional core facility available to all researchers without restriction. The ABI 3100 DNA sequencer will be sited in and operated by trained staff of the Core Facility. The 16 channels automated ABI 3100 instrument will replace an existing single channel ABI 310 DNA sequencer currently used by the Protein and Nucleic Acid Core Facility since 1999. Steadily increasing sample submissions due to markedly enhanced funding by and expansion in the number of Medical College investigators has exceeded the maximum capacity of the existing ABI 310 instrument. The proposed ABI 3100 DNA sequencer possesses a sample capacity that exceeds projected institutional needs for the foreseeable future yet retains the format and chemistry employed by the existing instrument and similar dedicated ABI 310 sequencers used by several Departments and groups, thus allowing seamless transitions for investigators using the latter instruments who choose to use the Protein and Nucleic Acid Core Facility. In addition, the proposed instrument will allow longer reads than currently available on the ABI 310 instrument.