REGULATION OF COLLAGENASE ACTIVITY IN THE UTERUS

子宫内胶原酶活性的调节

• 批准号: 2194883
• 负责人: JOHN J JEFFREY
• 金额: $ 24.65万
• 依托单位: ALBANY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2194883
• 关键词: biological signal transduction; birth; cell membrane; collagen; collagenase; connective tissue metabolism; enzyme mechanism; extracellular matrix; genetic regulatory element; genetic transcription; genetic translation; glucocorticoids; hormone regulation /control mechanism; human tissue; laboratory rat; molecular cloning; myometrium; organ culture; postpartum; pregnancy; progesterone; protein biosynthesis; receptor expression; second messengers; serotonin; serotonin receptor; tissue /cell culture; uterus

DESCRIPTION (Adapted from the applicant's abstract): The long term goals of this application are to define the mechanisms by which the pregnant and post-partum uterus regulates the metabolism of collagen. This protein is absolutely required by the uterus for the structural integrity of the organ during pregnancy; thus it is necessary to precisely regulate the amount and distribution of collagen in the tissue during gestation. After delivery, the protein is degraded during the general involution of the uterus which occurs in all mammals. The degradation of collagen in the uterus requires the action of a specific collagenase whose function it is to catalyze the crucial cleavages in collagen which initiate the process by which the protein is removed from the extracellular matrix. The physiologic processes by which the appearance of these molecules in the uterus is regulated will be studied in cell culture systems and in explant cultures. The nature of the mechanisms whereby collagenase production and activity can be induced, modulated in situ, and terminated when no longer required, will be studied. Such mechanisms involve the physiologic activities of important gestational hormones such as progesterone and the glucocorticoids, as well as a newly re-discovered hormone, serotonin. The results of these investigations should provide fundamental information regarding the mechanisms by which collagen degradation is accomplished in the uterus. It is hoped that by understanding the molecules involved in these processes and the way in which their biological activity is regulated, normal pregnancy and labor can be better understood and more rationally managed.

描述(改编自申请人的摘要)：长期目标 这一应用的目的是定义怀孕和怀孕的机制 产后子宫调节胶原蛋白的代谢。这种蛋白质是 子宫对器官结构的完整性是绝对必要的 在怀孕期间；因此有必要精确地调节数量和 妊娠期间胶原蛋白在组织中的分布。在分娩之后， 蛋白质在子宫的一般退缩过程中被降解， 发生在所有哺乳动物身上。子宫中胶原的降解需要 一种特定胶原酶的作用，其功能是催化 胶原蛋白中的关键裂解，它启动了 蛋白质从细胞外基质中去除。生理学 这些分子在子宫中出现的过程是 调控将在细胞培养系统和外植体培养中进行研究。 胶原酶产生和活性的机制的本质 可以在不再需要时被诱导、原位调制和终止， 将会被研究。这种机制涉及到人体的生理活动。 重要的妊娠激素，如孕酮和 糖皮质激素，以及一种新发现的激素--5-羟色胺。这个 这些调查的结果应提供基本信息 关于胶原蛋白在体内降解的机制 子宫。人们希望通过了解参与其中的分子 这些过程和它们的生物活性是 规范、正常的怀孕和分娩可以更好地理解和更多地 理性地管理。