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IMPROVED APPROACH FOR IDENTIFYING EXPRESSED SEQUENCES

IMPROVED APPROACH FOR IDENTIFYING EXPRESSED SEQUENCES
识别表达序列的改进方法
批准号：
2209092
负责人：
SHERMAN Morton WEISSMAN
金额：
$16.33万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
项目状态：
已结题
起止时间：
1993-04-01 至 1995-03-31
来源：
https://reporter.nih.gov/project-details/2209092
关键词：
artificial chromosomes complementary DNA fusion gene gene expression genetic library genetic promoter element genetic techniques high performance liquid chromatography human genetic material tag human tissue messenger RNA molecular cloning nucleic acid hybridization nucleic acid sequence polymerase chain reaction

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中文摘要
翻译
我们建议发展cDNA片段文库的制备方法
英文摘要
We propose to develop methods for preparing libraries of cDNA fragments derived from sequences adjacent to the cap site of mRNAs. These methods will be applied to generate 5' end cDNA libraries from a number of human sources. Methods will be developed to select directly from these libraries those cDNAs encoded by large DNA fragments such as YACs without the need to purify the YAC DNA from the host yeast sequences. These methods would allow selection of cDNAs from arrays of YACs. In addition, the usefulness of the 5' end libraries for obtaining promoter sequences and preparing full-length cDNAs will be evaluated. These libraries will provide an improved approach for detecting expressed sequences and positional cloning of disease genes.
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会议论文
Cytokines and lineage choice in hematopoietic precursors
  • 批准号：
    8613792
  • 项目类别：
  • 资助金额：
    $28.97万
  • 财政年份：
    2013
  • 负责人：
    SHERMAN Morton WEISSMAN
  • 依托单位：
    YALE UNIVERSITY
Cytokines and lineage choice in hematopoietic precursors
  • 批准号：
    8735141
  • 项目类别：
  • 资助金额：
    $28.97万
  • 财政年份：
    2013
  • 负责人：
    SHERMAN Morton WEISSMAN
  • 依托单位：
    YALE UNIVERSITY
Transcriptome & Methylome Analysis of Single Cells
  • 批准号：
    8133938
  • 项目类别：
  • 资助金额：
    $19.87万
  • 财政年份：
    2010
  • 负责人：
    SHERMAN Morton WEISSMAN
  • 依托单位：
    YALE UNIVERSITY
Transcriptome & Methylome Analysis of Single Cells
  • 批准号：
    7990042
  • 项目类别：
  • 资助金额：
    $24.83万
  • 财政年份：
    2010
  • 负责人：
    SHERMAN Morton WEISSMAN
  • 依托单位：
    YALE UNIVERSITY
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