GENETICS OF HEDGEHOG AND ADJACENT LOCI OF DROSOPHILA

在这项拨款中提出的研究解决了基因如何 控制发育中胚胎的图案形成过程。这 研究将确定果蝇刺猬基因在 建立段旁边界，这是两个边界之一 在分段过程中建立的发育领域边界 果蝇早期胚胎。刺猬的表达方式，如 通过免疫组织化学判断，将其与其他 参与这一过程的片段极性基因，既在 胚胎发生和维持过程中这一边界的建立 在想象中的圆盘里。对刺猬的管制 表达，无论是在建立和维持其空间表达 模式(根据其RNA和蛋白质产物的分布来判断) 关于刺猬RNA剪接的发育规律，Will 被调查。最后，刺猬产品的性质，以及 确定该基因功能的可能作用，将是 通过分离和测序来自于 刺猬的轨迹。

The research proposed in this grant addresses the question of how genes control the process of pattern formation in developing embryos. This research will determine the role of the hedgehog gene of Drosophila in establishing the parasegmental boundary, which is one of two developmental field boundaries established during segmentation of the early Drosophila embryo. The pattern of expression of hedgehog, as judged by immunohistochemistry, will be compared with that of other segment polarity genes involved in this process, both during the establishment of this boundary in embryogenesis and in its maintenance in the imaginal discs. Controls on the regulation of hedgehog expression, both in establishing and maintaining its spatial expression pattern (as judged by the distribution of its RNA and protein products) and on the developmental regulation of splicing of the hedgehog RNA, will be investigated. Finally, the nature of the hedgehog product, and the identification of possible roles of the gene's function, will be identified by isolation and sequencing of complete cDNAs from the hedgehog locus.