GENETICS OF HEDGEHOG AND ADJACENT LOCI OF DROSOPHILA

刺猬与果蝇邻近位点的遗传学

• 批准号: 3469394
• 负责人: JAMES P MOHLER
• 金额: $ 7.11万
• 依托单位: BARNARD COLLEGE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3469394
• 关键词: Drosophilidae; alleles; autoradiography; body regions; chromosomes; congenital disorders; developmental genetics; gel electrophoresis; gene complementation; gene expression; gene mutation; genes; genetic mapping; genetic regulation; histogenesis; metamorphosis; molecular cloning; molecular genetics; neoplasm /cancer; nonmammalian vertebrate embryology; nucleic acid hybridization; nucleic acid probes; temperature sensitive mutant; tissue mosaicism

This work will investigate the role genes play in the specification of the pattern of the embryo of Drosophila melanogaster. This will involve the characterization, in particular, of the hedgehog gene locus, which is a segmental polarity class locus. This work will characterize the spatial and temporal requirements for the hedgehog gene, both embryonically and post-embryonically, by use of genetic mosaics and temperature sensitive mutations. This work also characterize the molecular architecture of the hedgehog locus and the spatial and temporal expression of RNA transcripts from this locus. In addition, this work will characterize genetically the chromosomal region around the hedgehog locus, which is known already to contain another two pattern specification loci. The understanding of how this gene and others disrupt the normal pattern of the Drosophila embryo will provide more insight into the role genes play in the embryogenesis of developing organisms, and may be applicable to an understanding of human development. This work will also provide insight into the mechanisms of gene regulation and therefore provide information valuable to understanding those disease processes, such as cancer, in which aberrant regulation of certain genes may lead to a pathological problem.

这项工作将调查的作用基因发挥的规格 黑腹果蝇胚胎的模式。 这 会涉及到对刺猬的刻画 基因座，其是片段极性类基因座。 这项工作 将描述空间和时间的要求， hedgehog基因，胚胎和胚胎后，通过使用 基因嵌合体和温度敏感性突变。 这 工作还表征了 hedgehog基因座与RNA的时空表达 这个基因座的转录本 此外，这项工作将 从遗传学上描述了 hedgehog基因座，已知含有另外两个 模式特化基因座 了解这种基因和其他基因如何破坏正常的 果蝇胚胎的模式将提供更多的洞察力， 基因在发育生物体的胚胎发生中所起的作用， 并可能适用于人类发展的理解。 这项工作也将提供深入了解基因的机制， 法规，因此提供有价值的信息， 了解这些疾病的过程，比如癌症， 某些基因的异常调节可能导致病理性的 问题.