GENETICS OF HEDGEHOG AND ADJACENT LOCI OF DROSOPHILA

刺猬与果蝇邻近位点的遗传学

• 批准号: 3322584
• 负责人: JAMES P MOHLER
• 金额: $ 11.97万
• 依托单位: BARNARD COLLEGE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3322584
• 关键词: Drosophilidae; RNA splicing; alleles; body regions; developmental genetics; embryogenesis; gel electrophoresis; gene expression; gene mutation; genetic mapping; genetic regulation; histogenesis; invertebrate embryology; metamorphosis; molecular cloning; northern blottings; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; tissue mosaicism

The research proposed in this grant addresses the question of how genes control the process of pattern formation in developing embryos. This research will determine the role of the hedgehog gene of Drosophila in establishing the parasegmental boundary, which is one of two developmental field boundaries established during segmentation of the early Drosophila embryo. The pattern of expression of hedgehog, as judged by immunohistochemistry, will be compared with that of other segment polarity genes involved in this process, both during the establishment of this boundary in embryogenesis and in its maintenance in the imaginal discs. Controls on the regulation of hedgehog expression, both in establishing and maintaining its spatial expression pattern (as judged by the distribution of its RNA and protein products) and on the developmental regulation of splicing of the hedgehog RNA, will be investigated. Finally, the nature of the hedgehog product, and the identification of possible roles of the gene's function, will be identified by isolation and sequencing of complete cDNAs from the hedgehog locus.

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