GENETICS OF HEDGEHOG AND ADJACENT LOCI OF DROSOPHILA

刺猬与果蝇邻近位点的遗传学

• 批准号: 2332245
• 负责人: JAMES P MOHLER
• 金额: $ 9.13万
• 依托单位: BARNARD COLLEGE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2332245
• 关键词: Drosophilidae; RNA splicing; alleles; body regions; developmental genetics; embryogenesis; gel electrophoresis; gene expression; gene mutation; genetic mapping; genetic regulation; histogenesis; invertebrate embryology; metamorphosis; molecular cloning; northern blottings; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; tissue mosaicism

The research proposed in this grant addresses the question of how genes control the process of pattern formation in developing embryos. This research will determine the role of the hedgehog gene of Drosophila in establishing the parasegmental boundary, which is one of two developmental field boundaries established during segmentation of the early Drosophila embryo. The pattern of expression of hedgehog, as judged by immunohistochemistry, will be compared with that of other segment polarity genes involved in this process, both during the establishment of this boundary in embryogenesis and in its maintenance in the imaginal discs. Controls on the regulation of hedgehog expression, both in establishing and maintaining its spatial expression pattern (as judged by the distribution of its RNA and protein products) and on the developmental regulation of splicing of the hedgehog RNA, will be investigated. Finally, the nature of the hedgehog product, and the identification of possible roles of the gene's function, will be identified by isolation and sequencing of complete cDNAs from the hedgehog locus.

这项研究提出了一个问题，即基因如何 控制胚胎发育过程中图案的形成。 这 研究将确定果蝇刺猬基因在其中的作用 建立准分段边界，这是两个 发展领域的边界建立在分割的 果蝇早期胚胎 刺猬的表达模式，如 通过免疫组化判断，将其与其他 片段极性基因参与这一过程，无论是在 在胚胎发生和维持过程中这种边界的建立 在想象盘里 对刺猬的调控 表达，无论是在建立和维持其空间表达 模式（通过其RNA和蛋白质产物的分布来判断） 以及刺猬RNA剪接的发育调控， 追究 最后，刺猬产品的性质，以及 鉴定基因功能的可能作用，将是 通过分离和测序来自该细胞的完整cDNA来鉴定。 刺猬基因座

项目成果

Isolation and cloning of a Drosophila homolog to the mammalian RACK1 gene, implicated in PKC-mediated signalling.

• DOI: 10.1016/s0167-4889(97)00079-7
• 发表时间: 1997-08
• 期刊: Biochimica et biophysica acta
• 作者: K. Vani;G. Yang;J. Mohler