ANTICANCER AGENTS FROM TERRESTRIAL PLANTS

来自陆生植物的抗癌剂

• 批准号: 5209089
• 负责人: JOSEPH J HOFFMANN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5209089
• 关键词: SCID mouse; X ray crystallography; agriculture; antineoplastics; chemical structure function; chromatography; cooperative study; drug screening /evaluation; laboratory mouse; mass spectrometry; nuclear magnetic resonance spectroscopy; plant extracts

This Laboratory Program is part of a major group in the discovery of novel antitumor agents from natural sources. It is dedicated to terrestrial plants as potential sources of novel antitumor agents. Plants will primarily be acquired from the semi-arid U.S. Southwest and Western United States. A diversity of plant families and genera will be sought in order to maximize the chemical diversity available in the plant kingdom, thereby increasing the chances for positive results during the course of this investigation. An additional added criterion will be to search out rare and previously unexplored plant species. Once the plant materials have been received, they will be processed to provide crude materials for antitumor testing in a variety of the group's in vitro screens. Sufficiently active plants will be tested also for in vivo activity and subsequently subjected to bioassay directed fractionation and isolation and characterization of the active component(s). Approximately 400 plants per year (800 extracts) will be sent to Wayne State University for assay. Subsequent fractionation and purification of lead extracts, estimated at 2% or 8 plants will generate another 100- 200 extracts. Also, we presently have about 30 leads whose fractionation will begin upon initiation of the Program. This proposal outlines a workplan for analyzing selected species of the U.S. Southwest and West as potential sources of naturally-occurring antitumor drugs or synthons. This effort will also require plans for scaling-up the production of these compounds, either through range management or the development of specialty cash crops. This research component will include the training of graduate students and post-doctoral fellows in the growing and processing of plant materials, the isolation and identification of the active compounds, and large scale production of these specialty chemicals. In this manner the results of this research effort will add to the technology required for the development of sustainable agrisystems for arid and semi-arid lands.

这个实验室计划是一个主要小组的一部分，在发现 天然来源的新型抗肿瘤剂。 它致力于 陆生植物作为新型抗肿瘤药物的潜在来源。 植物将主要从半干旱的美国西南部收购， 美国西部。 植物科和属的多样性将是 为了最大限度地提高植物中的化学多样性， 王国，从而增加了积极成果的机会， 调查的过程。 另一个附加标准是 寻找以前未被发现的稀有植物物种。 一旦植物 材料已经收到，他们将被加工，以提供原油， 材料进行了多种组的体外抗肿瘤试验 卡位 还将对足够活性的植物进行体内试验 活性，随后进行生物测定指导的分级分离 以及活性组分的分离和表征。 每年大约有400株植物（800种提取物）将被送往韦恩 州立大学进行检测。 随后的分馏和纯化 铅提取物中，估计2%或8株植物将再产生100- 200个提取物。 此外，我们目前有大约30个线索， 将在计划启动时开始。该提案概述了 分析美国西南部和西部选定物种的工作计划 作为天然抗肿瘤药物或合成子的潜在来源。 这一努力还需要制定计划， 这些化合物，无论是通过范围管理或发展， 特色经济作物 这项研究将包括培训 研究生和博士后研究员在不断增长的， 植物材料的加工，分离和鉴定 活性化合物，以及大规模生产这些特殊的 化学品 通过这种方式，这项研究工作的结果将增加 发展可持续农业系统所需的技术 干旱和半干旱的土地。