ANTICANCER AGENTS FROM TERRESTRIAL PLANTS

该实验室计划是一个主要研究小组的一部分 天然来源的新型抗肿瘤药物。它致力于 陆生植物作为新型抗肿瘤药物的潜在来源。 植物将主要从半干旱的美国西南部和 美国西部。植物科和属的多样性将是 寻求最大限度地增加植物中可用的化学多样性 王国，从而增加了在 这起调查的过程。另一项增加的标准是 找出稀有和以前未被探索过的植物物种。一旦工厂 材料已经收到，它们将被加工以提供原油 用于抗肿瘤试验的材料在该小组的各种体外 屏幕。足够活跃的植物也将在体内进行测试 活性，然后进行生物测定定向分级 活性成分的分离和鉴定(S)。 每年大约400种植物(800种精华)将被送往韦恩 州立大学进行化验。后续分馏和提纯 在铅萃取物中，估计有2%或8种植物将产生另外100- 200个精华。此外，我们目前有大约30个线索，他们的分部 将在计划启动时开始。这份提案概述了一个 分析美国西南部和西部部分物种的工作计划 作为天然抗肿瘤药物或合成子的潜在来源。 这一努力还需要制定扩大生产的计划 这些化合物，无论是通过范围管理还是通过开发 特产经济作物。这一研究部分将包括培训 研究生和博士后研究员的成长和 植物原料的加工、分离和鉴定 活性化合物及其专用料的规模化生产 化学制品。以这种方式，这项研究工作的结果将添加 发展可持续农业系统所需的技术 对于干旱和半干旱的土地。

This Laboratory Program is part of a major group in the discovery of novel antitumor agents from natural sources. It is dedicated to terrestrial plants as potential sources of novel antitumor agents. Plants will primarily be acquired from the semi-arid U.S. Southwest and Western United States. A diversity of plant families and genera will be sought in order to maximize the chemical diversity available in the plant kingdom, thereby increasing the chances for positive results during the course of this investigation. An additional added criterion will be to search out rare and previously unexplored plant species. Once the plant materials have been received, they will be processed to provide crude materials for antitumor testing in a variety of the group's in vitro screens. Sufficiently active plants will be tested also for in vivo activity and subsequently subjected to bioassay directed fractionation and isolation and characterization of the active component(s). Approximately 400 plants per year (800 extracts) will be sent to Wayne State University for assay. Subsequent fractionation and purification of lead extracts, estimated at 2% or 8 plants will generate another 100- 200 extracts. Also, we presently have about 30 leads whose fractionation will begin upon initiation of the Program. This proposal outlines a workplan for analyzing selected species of the U.S. Southwest and West as potential sources of naturally-occurring antitumor drugs or synthons. This effort will also require plans for scaling-up the production of these compounds, either through range management or the development of specialty cash crops. This research component will include the training of graduate students and post-doctoral fellows in the growing and processing of plant materials, the isolation and identification of the active compounds, and large scale production of these specialty chemicals. In this manner the results of this research effort will add to the technology required for the development of sustainable agrisystems for arid and semi-arid lands.