Single particle imaging: x-ray imaging of individual dynamic biomolecules

单粒子成像：单个动态生物分子的 X 射线成像

• 批准号: DE200101061
• 负责人: Dr Andrew Morgan
• 金额: $ 29.83万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-12-18 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE200101061
• 关键词: Single; particle; imaging; ray; individual

X-ray lasers produce powerful ultra-short pulses of light that can take temporal snap shots of small radiation-sensitive biological complexes. Thanks to superconducting technology, the next generation of x-ray lasers will be able to produce x-ray pulses at greater rates than ever before. But because of the sheer number of possible molecular configurations, these molecular movies will have only a small amount of data per frame, posing an enormous challenge for current imaging methods. I aim to meet this challenge by developing an innovative multi-conformational image reconstruction algorithm. This will provide a new window into the molecular dynamics of biological systems, the building blocks of life, and enable rational drug design.

X 射线激光器产生强大的超短光脉冲，可以对小型辐射敏感生物复合物进行瞬时快照。得益于超导技术，下一代 X 射线激光器将能够以比以往更高的速率产生 X 射线脉冲。但由于可能的分子构型数量巨大，这些分子电影每帧只有少量数据，这对当前的成像方法构成了巨大的挑战。我的目标是通过开发一种创新的多构象图像重建算法来应对这一挑战。这将为了解生物系统的分子动力学（生命的组成部分）提供一个新的窗口，并实现合理的药物设计。