MOUSE GENOME MAP WITH BACTERIAL ARTIFICIAL CHROMOSOMES

带有细菌人工染色体的小鼠基因组图谱

基本信息

  • 批准号:
    2209163
  • 负责人:
  • 金额:
    $ 35.32万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1993
  • 资助国家:
    美国
  • 起止时间:
    1993-09-01 至 1996-08-31
  • 项目状态:
    已结题

项目摘要

We have developed a bacterial F-factor based system for cloning large fragments of mammalian DNA in E coli (the BAC system). The primary aims of this proposal are to construct a genomic library of the mouse in the BAC vector and to use these clones for physical mapping of the mouse genome. Previous work with human genome BAC vectors indicates that the large human inserts are not chimeric, they are stable, representative, and are relatively easy to manipulate. The mouse library will consist of arrayed bacterial clones with average insert sizes of approximately 175kb, and will represent 4-5 times coverage of the mouse genome. The clones will be rendered onto high density filters for colony hybridization, and will be pooled to permit library screening by PCR. We will use a number of different approaches to identify clones corresponding to established physical and genetic landmarks. Initial mapping experiments will focus on regions of the mouse chromosome that are replete with markers. We will use the library to isolate clones originating from the proximal region of mouse chromosome 17, and establish "contigs", or groups of overlapping clones. We will also focus on other regions that have high densities of both genetically and physically mapped markers. These experiments will provide us with the opportunity to quickly verify the extent to which the library is representative and to demonstrate generalizable procedures for using BACs to generate detailed maps of any region in the genome. In addition, we will isolate BAC clones which contain the STSs developed by Eric Lander's group and by other groups which will provide virtually complete coverage of the mouse genome in BACs. We will also apply the techniques of fluorescent in situ hybridization to analysis of the mouse genome. We will carry out BAC hybridizations to metaphase chromosomes to map BACs and confirm mapping data established by other approaches.
我们开发了一种基于细菌f因子的克隆系统

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Bruce W. BIRREN其他文献

Bruce W. BIRREN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Bruce W. BIRREN', 18)}}的其他基金

Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations
推进基因组技术对抗传染病:绘制单细胞、个体宿主和全球人群的动态图
  • 批准号:
    10641432
  • 财政年份:
    2022
  • 资助金额:
    $ 35.32万
  • 项目类别:
The 200 mammals project: sequencing genomes by a novel cost-effective method, yielding a high resolution annotation of the human genome
200 只哺乳动物项目:通过一种新颖的经济有效的方法对基因组进行测序,产生人类基因组的高分辨率注释
  • 批准号:
    10087672
  • 财政年份:
    2020
  • 资助金额:
    $ 35.32万
  • 项目类别:
The 200 mammals project: sequencing genomes by a novel cost-effective method, yielding a high resolution annotation of the human genome.
200 只哺乳动物项目:通过一种新颖的经济有效的方法对基因组进行测序,产生人类基因组的高分辨率注释。
  • 批准号:
    9173645
  • 财政年份:
    2016
  • 资助金额:
    $ 35.32万
  • 项目类别:
Admin Core
管理核心
  • 批准号:
    10163674
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations
推进基因组技术对抗传染病:绘制单细胞、个体宿主和全球人群的动态图
  • 批准号:
    10610394
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Admin Core
管理核心
  • 批准号:
    10610398
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations
推进基因组技术对抗传染病:绘制单细胞、个体宿主和全球人群的动态图
  • 批准号:
    10470461
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations
推进基因组技术对抗传染病:绘制单细胞、个体宿主和全球人群的动态图
  • 批准号:
    10447900
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Infectious Disease Genomics: Pathogen Evolution, Emergence, and Host Interaction
传染病基因组学:病原体进化、出现和宿主相互作用
  • 批准号:
    8836963
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:
Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations
推进基因组技术对抗传染病:绘制单细胞、个体宿主和全球人群的动态图
  • 批准号:
    9919476
  • 财政年份:
    2014
  • 资助金额:
    $ 35.32万
  • 项目类别:

相似海外基金

Genetic mapping of the inflammatory adaption circuit in epithelial stem cells
上皮干细胞炎症适应回路的遗传图谱
  • 批准号:
    10713508
  • 财政年份:
    2023
  • 资助金额:
    $ 35.32万
  • 项目类别:
Genetic Mapping of Modifier Loci in a Mouse Model KCNB1 Encephalopathy
KCNB1 脑病小鼠模型修饰位点的遗传图谱
  • 批准号:
    10753301
  • 财政年份:
    2023
  • 资助金额:
    $ 35.32万
  • 项目类别:
Genetic mapping of a stress response and pro-longevity network in C. elegans and human cancer cells
线虫和人类癌细胞的应激反应和长寿网络的基因图谱
  • 批准号:
    469325
  • 财政年份:
    2022
  • 资助金额:
    $ 35.32万
  • 项目类别:
    Operating Grants
Genetic mapping of variable cardiac cell composition in the rat
大鼠可变心脏细胞组成的遗传图谱
  • 批准号:
    10301081
  • 财政年份:
    2021
  • 资助金额:
    $ 35.32万
  • 项目类别:
Genetic mapping of variable cardiac cell composition in the rat
大鼠可变心脏细胞组成的遗传图谱
  • 批准号:
    10458033
  • 财政年份:
    2021
  • 资助金额:
    $ 35.32万
  • 项目类别:
Dissecting the control of continuous cropping in strawberry using genetic mapping and gene editing
利用遗传作图和基因编辑剖析草莓连作的控制
  • 批准号:
    BB/W51083X/1
  • 财政年份:
    2021
  • 资助金额:
    $ 35.32万
  • 项目类别:
    Training Grant
Dissecting the control of continuous cropping in strawberry using genetic mapping and gene-editing
利用遗传图谱和基因编辑剖析草莓连作的控制
  • 批准号:
    2587574
  • 财政年份:
    2021
  • 资助金额:
    $ 35.32万
  • 项目类别:
    Studentship
NSF Postdoctoral Fellowship in Biology FY 2019: Leveraging Quantitative Genetic Mapping Data for Discovery of Novel Regulatory DNA in Solanum lycopersicum
2019 财年 NSF 生物学博士后奖学金:利用定量遗传图谱数据发现番茄中的新型调控 DNA
  • 批准号:
    1907088
  • 财政年份:
    2019
  • 资助金额:
    $ 35.32万
  • 项目类别:
    Fellowship Award
Genetic Mapping of Novel Molecular Players in Itch
瘙痒中新分子参与者的基因图谱
  • 批准号:
    9437883
  • 财政年份:
    2017
  • 资助金额:
    $ 35.32万
  • 项目类别:
High-resolution Genetic Mapping of Maternal Effects and Epistatic Suppressors of Congenital Heart Disease Risk
先天性心脏病风险的母体效应和上位抑制因子的高分辨率基因图谱
  • 批准号:
    9256806
  • 财政年份:
    2017
  • 资助金额:
    $ 35.32万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了