Advancing Genomic Technologies to Combat Infectious Disease: Mapping Dynamics within Single Cells, Individual Hosts, and Global Populations

推进基因组技术对抗传染病：绘制单细胞、个体宿主和全球人群的动态图

• 批准号: 10641432
• 负责人: Bruce W. BIRREN
• 金额: $ 138.47万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10641432
• 关键词: Cells; Clinical; Communicable Diseases; Drug resistance; Enrollment; Genomics; Incidence; Individual; Outcome; Patients; Population; Reporting; Research Personnel; Risk Factors; Ritonavir; Symptoms; Syndrome; Technology; Viral; Viral Genome; Viral load measurement; cohort; combat; genome sequencing; nirmatrelvir; prospective; virology

Project Summary/Abstract Clinical rebound following treatment with nirmatrelvir-ritonar has been reported. The incidence, virology, and clinical consequence of this syndrome are not clear. The investigators will conduct a study of nirmatrelvir-ritonavir rebound with the following objectives: Objective 1: Determine the incidence and risk factors for symptomatic and asymptomatic virologic rebound after nirmatrelvir-ritonavir therapy Objective 2: Determine the duration and magnitude of virologic rebound and contagiousness of symptomatic and asymptomatic rebound after nirmatrelvir-ritonavir therapy Objective 3: Estimate the incidence of treatment-emergent drug resistance after rebound Objective 4: Describe clinical outcomes with and without virologic rebound following nirmatrelvir-ritonavir therapy in a real-world setting By carrying out these objectives, the investigators will define the incidence of rebound after nirmatrelvir-ritonavir and characterize the associated syndrome using quantitative measurement of viral load, viral culture, and whole virus genome sequencing.

项目摘要/摘要 尼马瑞韦-利托纳治疗后的临床反弹已有报道。发病率， 这种综合征的病毒学和临床后果尚不清楚。调查人员将进行 尼马瑞韦-利托那韦反弹的研究目的如下： 目标1：确定有症状和无症状的发病率和危险因素 尼马瑞韦-利托那韦治疗后病毒学反弹 目标2：确定病毒学反弹的持续时间和幅度以及传染性 尼马瑞韦-利托那韦治疗后有症状和无症状的反弹 目的3：评估药物反弹后紧急耐药的发生率 目标4：描述有和没有病毒学反弹的临床结果 尼马瑞韦-利托那韦在真实环境中的治疗 通过实现这些目标，调查人员将定义反弹的发生后 尼马瑞韦-利托那韦及其相关综合征的定量研究 病毒载量、病毒培养和全病毒基因组测序。

项目成果

The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences.

整个醋酸杆菌属的基因组多样化：起源、机制和后果。

• DOI: 10.1093/gbe/evu225
• 发表时间: 2014-10-13
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Touchon M;Cury J;Yoon EJ;Krizova L;Cerqueira GC;Murphy C;Feldgarden M;Wortman J;Clermont D;Lambert T;Grillot-Courvalin C;Nemec A;Courvalin P;Rocha EP
• 通讯作者: Rocha EP

Genomic analysis of globally diverse Mycobacterium tuberculosis strains provides insights into the emergence and spread of multidrug resistance.

• DOI: 10.1038/ng.3767
• 发表时间: 2017-03
• 期刊: Nature genetics
• 影响因子: 30.8
• 作者: Manson AL;Cohen KA;Abeel T;Desjardins CA;Armstrong DT;Barry CE 3rd;Brand J;TBResist Global Genome Consortium;Chapman SB;Cho SN;Gabrielian A;Gomez J;Jodals AM;Joloba M;Jureen P;Lee JS;Malinga L;Maiga M;Nordenberg D;Noroc E;Romancenco E;Salazar A;Ssengooba W;Velayati AA;Winglee K;Zalutskaya A;Via LE;Cassell GH;Dorman SE;Ellner J;Farnia P;Galagan JE;Rosenthal A;Crudu V;Homorodean D;Hsueh PR;Narayanan S;Pym AS;Skrahina A;Swaminathan S;Van der Walt M;Alland D;Bishai WR;Cohen T;Hoffner S;Birren BW;Earl AM
• 通讯作者: Earl AM

Genomic characterization of recurrent mold infections in thoracic transplant recipients.

胸腔移植受者复发性霉菌感染的基因组特征。

• DOI: 10.1111/tid.12935
• 发表时间: 2018
• 期刊: Transplant infectious disease : an official journal of the Transplantation Society
• 作者: Messina,JuliaA;Wolfe,CameronR;Hemmersbach-Miller,Marion;Milano,Carmelo;Todd,JamieL;Reynolds,John;Alexander,BarbaraD;Schell,WileyA;Cuomo,ChristinaA;Perfect,JohnR
• 通讯作者: Perfect,JohnR

Field-deployable viral diagnostics using CRISPR-Cas13.

• DOI: 10.1126/science.aas8836
• 发表时间: 2018-04-27
• 期刊: Science (New York, N.Y.)
• 作者: Myhrvold C;Freije CA;Gootenberg JS;Abudayyeh OO;Metsky HC;Durbin AF;Kellner MJ;Tan AL;Paul LM;Parham LA;Garcia KF;Barnes KG;Chak B;Mondini A;Nogueira ML;Isern S;Michael SF;Lorenzana I;Yozwiak NL;MacInnis BL;Bosch I;Gehrke L;Zhang F;Sabeti PC
• 通讯作者: Sabeti PC

The Anopheles coluzzii microbiome and its interaction with the intracellular parasite Wolbachia.

库氏按蚊微生物组及其与细胞内寄生虫沃尔巴克氏体的相互作用。

• DOI: 10.1038/s41598-020-70745-0
• 发表时间: 2020
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Straub,TimothyJ;Shaw,WRobert;Marcenac,Perrine;Sawadogo,SimonP;Dabiré,RochK;Diabaté,Abdoulaye;Catteruccia,Flaminia;Neafsey,DanielE
• 通讯作者: Neafsey,DanielE