AGGRESSIVE BEHAVIOR AND ANDROGEN SURGES

攻击性行为和雄激素激增

• 批准号: 2242738
• 负责人: STEVEN M PHELPS
• 金额: $ 1.3万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-10 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2242738
• 关键词: AGGRESSIVE; BEHAVIOR; ANDROGEN; SURGES

Any attempt to gain insight into the biological substrates of violence, which the National Center for Disease Control has labeled the most prominent health concern afflicting Americans, ought to address interactions of environmental and biological determinants, and do so in a way that suggests the generality of these mechanisms. Transient testosterone (T) surges that follow successful aggression are hypothesized to act as positive reinforcers and as rapid activators of aggression through actions on membrane steroid receptors. Exogenously administered T-surges, successful aggression in intact animals, and successful aggression in castrates with tonic-release T-implants will be tested for reinforcement properties in an operant conditioning paradigm. Measurement of aggressive responsiveness to intruding males shortly after administration of exogenous T-surges will be used to test rapid activating effects of T. Membrane binding sites for T and its metabolites, estradiol and 5 alpha-dihydrotestosterone, will be sought and characterized using conjugates of the steroids to bovine serum albumin and quantitative autoradiography of cryostat sections of the forebrain. If regions of high expression are found and binding sites have the characteristics of receptors, the cDNA corresponding to those sites will be cloned. Cloning will employ insertion into a plasmid vector, expression in eukaryotic host cells, and several rounds of expression screening and retransfection, before isolating and sequencing insert DNA. Understanding mechanisms of aggression in the study organism, Anolis carolinensis, will facilitate future comparative study using a subgroup of its over 250 congeners.

任何试图深入了解暴力的生物学基础的尝试， 美国国家疾病控制中心称之为 困扰美国人的突出健康问题，应该解决 环境和生物决定因素的相互作用，并在 这种方式表明了这些机制的普遍性。瞬态 成功攻击后睾丸激素（T）激增， 假设作为积极的免疫和快速激活剂， 通过对膜类固醇受体的作用进行攻击。外源 给予T波，在完整动物中成功攻击， 成功的侵略阉割与强直释放T型植入物将 在操作性条件反射范例中测试强化特性。 测量入侵后不久的攻击性反应 外源性T峰的施用将用于测试快速 T. T及其受体的膜结合位点 将寻找代谢产物雌二醇和5 α-双氢睾酮 并使用类固醇与牛血清的缀合物进行表征 白蛋白和定量放射自显影的冷冻切片的 前脑如果发现高表达的区域并且结合位点具有 受体的特征，对应于这些位点的cDNA 将被克隆。克隆将采用插入到质粒载体中， 在真核宿主细胞中的表达，以及几轮表达 筛选和再转染，然后分离和测序插入DNA。 了解研究生物体中的攻击机制，Anolis carolinensis，将促进未来的比较研究，使用亚组 超过250种同类物。