SEX HORMONE EFFECTS ON LIPOPROTEIN SPECIATION

年，美国将有4300万妇女年满50岁。 冠状动脉疾病（CAD）将是导致冠心病的主要原因。 死亡在这个群体中。因此，了解风险势在必行 导致CAD病理生理学的因素。其中， 脂蛋白的改变起主要作用。然而， 了解了各种形式的高密度和低密度脂蛋白（HDL 和LDL）及其调节。 因此，本研究的目的是阐明 内源性和外源性雌激素、孕激素和雄激素对物种形成的影响 育龄妇女和绝经后妇女的HDL和LDL组成。 为了实现这一目标，将在三组妇女中进行研究： 1)月经周期正常的女性[给药前后 促性腺激素释放激素（GnRH）类似物] ; 2）高雄激素女性 生殖年龄（在给予GnRH类似物之前和之后， 和不添加雌激素] ;和3）绝经后妇女[一组 没有外源性类固醇，一组只接受雌激素，第三组只接受雌激素。 雌激素加雷公藤多甙组]。所有研究中的女性 促性腺激素、性类固醇（雌激素、雄激素、孕酮）和性 激素结合球蛋白，通过放射免疫测定法测定。这些测量 将与新发现的本地 通过选择的亲和免疫吸附评估的HDL种类， 免疫测定，并与LDL的量化形态状态评估 通过梯度超离心和电子显微镜。这些 相关性将为性别之间的关系提供新的见解， 类固醇激素环境，年龄和脂蛋白，并应借给更大 了解女性CAD的病因、治疗和预防。

Forty-three million women will reach the age of 50 in the United States in this decade, and coronary artery disease (CAD) will be the leading cause of death in this group. Therefore, it is imperative to understand the risk factors which contribute to the pathophysiology of CAD. Among these, alterations in lipoproteins play a major role. However, much remains to be learned about the various forms of high and low density lipoproteins (HDL and LDL) and their regulation. Therefore, the goal of this study is to elucidate the effects of endogenous and exogenous estrogens, progestins and androgens on speciation of HDL and LDL composition in reproductive age and postmenopausal women. To achieve this goal, studies will be performed in three groups of women: 1) women with normal menstrual cycles [before and after administration of a gonadotropin releasing hormone (GnRH) analog] ; 2) hyperandrogenic women of reproductive age (before and after administration of a GnRH analog with and without added estrogen] ; and 3) postmenopausal women [one group without exogenous steroids, one group receiving estrogen only, and a third group receiving estrogen plus progestin]. Women in all studies will have gonadotropins, sex steroids (estrogens, androgens, progesterone), and sex hormone binding globulin, measured by radioimmunoassay. These measurements will be correlated with the distribution of newly identified native species of HDL as assessed by selected affinity immunosorption and immunoassays, and with the quantized speciation state of LDL as assessed by gradient ultracentrifugation and electron microscopy. These correlations will provide new insights into the relationships between sex steroid hormone milieu, age and lipoproteins, and should lend to greater understanding of the etiology, treatment and prevention of CAD in women.