A thermodynamic pathway to intracellular delivery

细胞内传递的热力学途径

• 批准号: DP200101860
• 负责人: Prof Majid Ebrahimi Warkiani
• 金额: $ 35.43万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-07-06 至 2023-11-05
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200101860
• 关键词: thermodynamic; pathway; intracellular; delivery

Cells transmit information through molecules. By delivering foreign molecules into cells, such as DNA and proteins, it is possible to engineer and reprogram cells just like a computer. This proposal aims to develop a novel microfluidic device for intracellular delivery. The device will work by exposing cells to rapid thermal shock to generate transient disruptions in cell membranes and thereby enable influx of foreign molecules into cells. To understand how the method can be optimized, the thermodynamic pathway of membrane disruption will be investigated at a single cell level. The methods and insights arising from this project could eventually lead to novel, patentable and lower-cost health technologies.

细胞通过分子传递信息。通过将外源分子（如DNA和蛋白质）输送到细胞中，就可以像计算机一样对细胞进行工程设计和重新编程。该方案旨在开发一种用于细胞内递送的新型微流体装置。该装置将通过将细胞暴露于快速热冲击来工作，以在细胞膜中产生瞬时破坏，从而使外源分子流入细胞。为了了解如何优化该方法，将在单细胞水平上研究膜破坏的热力学途径。该项目产生的方法和见解最终可能导致新颖的，可申请专利的和成本较低的健康技术。