PCB, PCDD, PCDF, AND PAH EXPOSURE FROM HAZARDOUS WASTE AMONG MOHAWK INDIANS

莫霍克印第安人接触危险废物的 PCB、PCDD、PCDF 和 PAH

• 批准号: 3840813
• 负责人: EDWARD F FITZGERALD
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3840813
• 关键词: Native Americans; air sampling /monitoring; biomarker; blood chemistry; breast feeding; breath tests; carbopolycyclic compound; cytochrome P450; dioxins; embryo /fetus toxicology; environmental contamination; environmental toxicology; enzyme induction /repression; epidemiology; feces analysis; halobiphenyl /halotriphenyl compound; human milk; human pregnant subject; human subject; industrial waste; infant human (0-1 year); mother /embryo /fetus nutrition; nutrition related tag; polychlorodibenzofuran; pregnancy circulation; soil pollution; soil sampling; statistics /biometry; toxin metabolism; umbilical cord; urinalysis; waste disposal; water pollution; water sampling /testing; water supply

An epidemiologic study is proposed of Mohawk women and infants to test the hypotheses that exposure to polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and polynuclear aromatic hydrocarbons (PAHs) from a nearby Superfund hazardous waste site elevates body burdens and affects the cytochrome P-450-dependent mixed function monooxygenase system. Exposure and body burdens will be assessed by: 1) estimating cumulative maternal dose from ingestion, inhalation, and dermal contact; 2) measuring concentrations of 68 routine PCB congeners in serum and urine, and, if lactating, of an additional 12 coplanar PCB congeners and of 34 PCDD/F congeners and 15 PAHs in breast milk; and 3) measuring infant concentrations of the PCB congeners in cord serum and urine and output of the PCDD/F congeners in feces. P-450IA2 activity in the mothers and infants will be assessed through a caffeine breath test, a safe and noninvasive procedure that measures the rate at which labelled carbon dioxide is exhaled as an indirect monitor. Pregnant Mohawk women will be interviewed and invited to participate in maternal blood sampling during their second trimester and cord blood sampling at delivery. They will also be asked to provide blood, urine, and breast milk samples and to collect infant urine and feces in cloth diapers at 1, 3, and 6 months post-partum to investigate change in pollutant levels over time. Multivariable statistical techniques will be used: 1) to test for differences in the mean contaminant levels of mothers according to cumulative dose and of infants according to breast-feeding status; 2) to relate breast milk pollutant concentrations to maternal and infant urinary levels and infant fecal output; and 3) to correlate P-450IA2 activity with levels of PCB and PCDD/F congeners and PAHs. A novel statistical technique based on summary scores will be used to "fingerprint" the waste site as a point source. The results will significantly enhance our understanding of how important classes of chemicals arising from hazardous waste bioaccumulate in human adults and infants and whether P-450IA2 induction is a sensitive biomarker of their early biologic effect.

一项流行病学研究提出了莫霍克妇女和婴儿，以测试 假设暴露于多氯联苯（PCBs）， 多氯二苯并对二恶英和多氯二苯并呋喃，以及 多环芳烃（PAHs）从附近的超级基金危险 废物场地提高了身体负担，并影响细胞色素P-450依赖的 混合功能单加氧酶系统 暴露和身体负担将是 通过以下方式进行评估：1）估计摄入的累积母体剂量， 吸入和皮肤接触; 2）测量68种常规 血清和尿液中的多氯联苯同系物，如果是哺乳期妇女， 共面PCB同源物和34 PCDD/F同源物和15多环芳烃的乳房 3）测量婴儿脐带中多氯联苯同系物的浓度 血清和尿液以及粪便中多氯二苯并对二恶英/多氯二苯并呋喃同系物的排出量。 P-450IA2 母亲和婴儿的活动将通过咖啡因来评估， 呼吸测试，一种安全和非侵入性的程序， 其标记的二氧化碳作为间接监测物呼出。 怀孕 莫霍克妇女将接受采访，并邀请参加孕产妇 在妊娠中期采集血液样本， 交付. 他们还将被要求提供血液，尿液和母乳 样本，并收集婴儿的尿液和粪便在布尿布在1，3， 产后6个月，调查污染物水平随时间的变化。 将使用多变量统计技术：1）检验 母亲的平均污染物水平的差异， 根据母乳喂养状况计算婴儿的累积剂量; 2） 母乳污染物浓度与母婴尿 水平和婴儿粪便排出量;和3）将P-450 IA 2活性与 多氯联苯和多氯二苯并对二恶英/多氯二苯并呋喃同系物和多环芳烃含量。 一种新的统计技术 根据总结分数将被用来“指纹”的废物网站作为一个 点源 结果将大大提高我们对 危险废物产生的化学品的重要类别 在成人和婴儿中的生物累积，以及P-450 IA 2诱导是否 这是它们早期生物效应的敏感生物标志物。