EXPRESSION OF THE PLASMODIAL NUTRIENT CHANNEL ON OOCYTES

卵母细胞上疟原虫营养通道的表达

• 批准号: 2057457
• 负责人: SANJAY A DESAI
• 金额: $ 6.77万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2057457
• 关键词: Plasmodium falciparum; Xenopus oocyte; adenosine triphosphate; antimalarial agents; erythrocyte membrane; erythrocytes; gene expression; human tissue; lactates; lipid bilayer membrane; membrane activity; membrane channels; membrane permeability; membrane reconstitution /synthesis; membrane transport proteins; messenger RNA; microinjections; nutrient intake activity; nutrition related tag; parasitism; potassium channel; second messengers; tissue /cell culture; vesicle /vacuole; voltage /patch clamp

This is a proposal to isolate and characterize the nutrient-permeable ion channel on the intraerythrocytic malaria parasite, P. falciparum. This channel was first described by the applicant and his colleagues [1, appendix] and may be essential for parasite survival because it permits the parasite to acquire amino acids and monosaccharides from the red blood cell (RBC). This channel was identified by using the patch clamp method to measure ion channel activity on the parasitophorous vacuole membrane (PVM), the outer of two membranes surrounding the parasite. The applicant now proposes to express this channel on Xenopus oocytes by microinjection of parasite mRNA. Expressed channels will then be used to measure K+, lactate, and ATP4- permeabilities, examine channel regulation, and investigate possible blockers. If expression on oocytes is unsuccessful, an alternative approach, reconstitution into lipid bilayers, will allow the same detailed characterization. These studies should address fundamental questions on parasite nutrient trafficking and may have profound implications for the treatment of malaria: an identified channel blocker may prevent nutrient acquisition and, therefore, kill parasites through starvation. The applicant has decided to focus his long-term research efforts on the mechanisms of plasmodial survival within the RBC. He has considerable experience with methods used to study membrane transport and will, with this award, develop a foundation in the methods of molecular biology.

这是一个分离和表征营养渗透离子的建议

项目成果

Parameters influencing the susceptibility of neonate mice to infection with Brugia pahangi.

影响新生小鼠对彭亨布鲁虫感染易感性的参数。

• 发表时间: 1983
• 期刊: The Journal of parasitology
• 作者: Furman,A;Ash,LR
• 通讯作者: Ash,LR