FEMTOSECOND PULSE SOURCE FOR NONLINEAR LASER MICROSCOPY

用于非线性激光显微镜的飞秒脉冲源

• 批准号: 2285470
• 负责人: FRANK W WISE
• 金额: $ 18.21万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2285470
• 关键词: biomedical equipment development; confocal scanning microscopy; lasers; solid state

DESCRIPTION: The long-term objective of this research is the development of a compact, robust, and user-friendly laser source for 2-photon- excited laser scanning microscopy. Femtosecond-duration pulses from a modelocked solid- state laser operating at wavelengths around 1.3 microns will be frequency- doubled to generate wavelength-tunable pulses in the wavelength range around 650 nanometers. The modelocked laser will be pumped by an array of laser diodes to reduce the size and cost of the source while improving its stability and reliability. Such a source will provide researchers in the biological and biophysical sciences much greater access to nonlinear laser microscopies while enhancing and expanding its capabilities. The source will be designed for compatibility with the large and growing base of laser scanning microscopes. The development of new and/or enhanced capabilities for microscopy will have a significant bearing on health. Improved means for visualizing submicron chemistry will aid the understanding of molecular processes in both normal and diseased states. Intracellular micropharmacology is one directly health-related example of nonlinear laser microscopy: Highly localized and specific treatments become possible by 2-photon uncaging of bioeffector molecules microscopy.

描述：本研究的长期目标是开发 一个紧凑的，强大的，用户友好的激光源，用于双光子， 激发激光扫描显微镜 飞秒脉冲 锁模固体激光器工作波长约为1.3 微米将被倍频，以产生波长可调的 波长范围在650纳米左右的脉冲。 的锁模的 激光将由激光二极管阵列泵浦，以减小尺寸 和成本，同时提高其稳定性和可靠性。 这样的来源将为生物和生物物理领域的研究人员提供 科学家更容易获得非线性激光显微镜， 加强和扩大其能力。 源将被设计为 为了与激光扫描的大型和不断增长的基础兼容， 显微镜. 开发新的和/或增强的显微镜功能将 对健康有重大影响。改进的可视化手段 亚微米化学将有助于理解分子过程 在正常和疾病状态下。细胞内微药理学是 一个与健康直接相关的非线性激光显微镜的例子： 高度局部化和特异性治疗成为可能，通过双光子 生物效应分子显微术的展开。