VIBRATIONAL SPECTROSCOPY OF PROTEIN NUCLEATION AND GROWTH

蛋白质成核和生长的振动光谱

• 批准号: 3755800
• 负责人: STEVEN H MORGAN
• 金额: --
• 依托单位: FISK UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755800
• 关键词: Raman spectrometry; X ray crystallography; aminoacid; analytical method; chemical bond resonance; chemical structure function; crystallization; infrared spectrometry; macromolecule; proteins; solubility; temperature; water solution

The inability to routinely grow single crystals of sufficient size and quality is a major roadblock to the wider application of protein crystallography. The development of more effective growth techniques requires a better understanding of the mechanism of protein crystallization. We proposed to apply some of the spectroscopic techniques used to investigate small molecule nucleation and growth to the study of the growth and nucleation of biological macromolecules Methodology will be developed using small molecule systems on which Raman studies of nucleation have been previously published. The applicability of infrared spectroscopy (GTIR-ATR) for detecting and identifying subnuclei at the very early stages of formation will also be determined. These techniques will be applied initially to amino acids, and then to peptides and proteins. Solubility data and super-saturation for proteins in H2O as a function of temperature, concentration of precipitating agent and pH will be obtained for systems studied. The use of temperature as a control parameter in protein crystal growth will also be investigated. Proteins suitable for temperature programmed growth experiments will be selected based upon either published solubility data or data obtained during the course of the proposed research.

不能常规地生长足够尺寸的单晶， 质量是蛋白质广泛应用的主要障碍 结晶学 开发更有效的生长技术 需要更好地理解蛋白质的作用机制 晶化 我们建议使用一些光谱技术 用于研究小分子的成核和生长， 生物大分子的生长和成核 使用小分子系统开发，在该小分子系统上拉曼研究成核 之前已经出版。 红外光谱的适用性 （GTIR-ATR）用于在非常早期的阶段检测和鉴定亚核 的形成也将确定。 这些技术将应用于 最初是氨基酸，然后是肽和蛋白质。 溶解度 数据和蛋白质在H2O中的过饱和度作为温度的函数， 沉淀剂的浓度和pH值将获得系统 研究了 温度作为蛋白质晶体的控制参数 还将研究增长。 适合温度的蛋白质 程序化生长实验将根据已发表的 溶解度数据或在建议的过程中获得的数据 research.