OPTICAL AND SPECTROSCOPIC STUDIES OF PROTEIN NUCLEATION AND GROWTH

蛋白质成核和生长的光学和光谱研究

• 批准号: 6239986
• 负责人: STEVEN H MORGAN
• 金额: $ 24.34万
• 依托单位: FISK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239986
• 关键词: Raman spectrometry; X ray crystallography; aminoacid; analytical method; chemical bond resonance; chemical structure function; crystallization; infrared spectrometry; macromolecule; optics; proteins; solubility; temperature; water solution

Efforts to develop improved techniques for the growth of protein crystals have been driven by the need to determine protein crystal structures for medical and pharmaceutical applications. The inability to routinely grow single crystals of sufficient size and quality is a major roadblock to the wider application of protein crystallography. We propose to investigate the use of temperature as a control parameter in the crystal growth of proteins and other biomolecules. Methodology will be developed using amino acid and simple peptide solutions. Proteins suitable for temperature programmed growth experiments will be selected based upon either published solubility data or data obtained during the course of the proposed research. We also propose to investigate the nonlinear optical response of amino acid, peptide and protein solutions near saturation. The purpose of the nonlinear optical investigation is to determine if there are changes in fast (100 fs - 100 ps) structural fluctuations which occur as the biomolecules begin to aggregate in the initial stages of crystal growth. It may then be possible to study the onset of nucleation by looking at the dynamics of these fluctuations during the crystal growth process.

努力开发蛋白质晶体生长的改进技术 受到确定蛋白质晶体结构的需求的驱动 医疗和制药应用。无法正常成长 足够尺寸和质量的单晶是实现这一目标的主要障碍 蛋白质晶体学的应用更加广泛。我们建议 研究使用温度作为晶体的控制参数 蛋白质和其他生物分子的生长。将制定方法论 使用氨基酸和简单的肽溶液。蛋白质适合 程序升温生长实验的选择基于 已发表的溶解度数据或在过程中获得的数据 拟议的研究。我们还建议研究非线性 氨基酸、肽和蛋白质溶液附近的光学响应 饱和。非线性光学研究的目的是 确定快速（100 fs - 100 ps）结构是否有变化 当生物分子开始聚集时发生的波动 晶体生长的初始阶段。那么也许可以研究 通过观察这些波动的动态来确定成核的开始 在晶体生长过程中。