DOMAIN STRUCTURE OF PLATELET INTEGRIN IIB-IIIA

血小板整合素 IIB-IIIA 的结构域

• 批准号: 2291749
• 负责人: KENNETH C. INGHAM
• 金额: $ 2万
• 依托单位: AMERICAN NATIONAL RED CROSS
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1996-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2291749
• 关键词: Escherichia coli; SDS polyacrylamide gel electrophoresis; calorimetry; chemical stability; divalent metal; gene expression; integrins; intermolecular interaction; ligands; peptide chemical synthesis; phase change; platelets; protein denaturation; protein folding; protein structure function; proteolysis; spectrometry; synthetic peptide; thermodynamics

Glycoprotein IIbIIIa is a heterodimeric integral membrane complex found on the surface of platelets and endothelial cells. A member of the integrin family of adhesion receptors, it plays an important role in blood clotting and wound healing. The goal of this research is to elucidate the structural and functional domain organization of IIbetaIIIalpha, to determine which parts of each subunit are independently folded and which parts are responsible for the various interactions. This will be accomplished through the preparation of fragments representing various parts of each subunit and examining their thermodynamic properties in comparison to those of the parent proteins through a combination of differential scanning calorimetry and spectroscopic methods. Those parts of each subunit which are involved in subunit interactions will be determined by evaluating the ability of the various fragments from one subunit to associate with the other subunit or its fragments. The interactions with divalent metal ions will be further characterized by measuring the effects of those ions on the stability of isolated domains and fragments. Knowledge of the domain structure of IIbetaIIIalpha will be helpful in understanding the molecular basis of diseases (such as Glanzmann's thrombasthenia) resulting from mutations in the genes coding for this receptor. Such knowledge will also serve as a guide to those who would attempt to use recombinant techniques to map the functions of this protein and other members of the integrin family.

糖蛋白IIbIIIa是一种异二聚体整体膜复合物