SHARED CIRCULAR DICHROMETER/FLUOROMETER

为研究重组蛋白质和片段的折叠特性，需要购买一台集成的圆形二色仪/荧光仪的经费。荷兰实验室内代表四个不同部门的四个主要用户和六个次要用户将受益于该仪器的提供。被选定进行研究的蛋白质范围很广，包括血液蛋白质及其受体、参与信号传递和肌动蛋白组织的细胞内蛋白质、细胞外基质蛋白质以及来自乳铁蛋白的防御素样抗菌肽。拟议的研究将更好地理解这些蛋白质在各自正常和异常生理中的作用，可能会导致开发出从癌症到阿尔茨海默氏症等各种疾病的改进治疗方法。

Funds are requested for purchase of an integrated circular dichrometer/fluorometer for the purpose of studying the folding properties of recombinant proteins and fragments. Four major users and six minor users representing four different departments within the Holland Laboratory will benefit from the availability of this instrument. A wide range of proteins are slated for study including blood proteins and their receptors, intracellular proteins involved in signalling and actin organization, proteins of the extracellular matrix, and a defensin-like antibacterial peptide derived from lactoferrin. The proposed studies will produce a better understanding of the role of these proteins in their respective normal and abnormal physiologies potentially leading to the development of improved therapies for a variety of diseases from cancer to Alzheimer's.