SHARED CIRCULAR DICHROMETER/FLUOROMETER

共享圆形二色计/荧光计

• 批准号: 6291973
• 负责人: KENNETH C. INGHAM
• 金额: $ 11万
• 依托单位: AMERICAN NATIONAL RED CROSS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291973
• 关键词: biomedical equipment purchase; circular dichroism; fluorimetry

Funds are requested for purchase of an integrated circular dichrometer/fluorometer for the purpose of studying the folding properties of recombinant proteins and fragments. Four major users and six minor users representing four different departments within the Holland Laboratory will benefit from the availability of this instrument. A wide range of proteins are slated for study including blood proteins and their receptors, intracellular proteins involved in signalling and actin organization, proteins of the extracellular matrix, and a defensin-like antibacterial peptide derived from lactoferrin. The proposed studies will produce a better understanding of the role of these proteins in their respective normal and abnormal physiologies potentially leading to the development of improved therapies for a variety of diseases from cancer to Alzheimer's.

要求提供资金购买一台综合圆二色/荧光计，以研究重组蛋白质和片段的折叠特性。代表荷兰实验室四个不同部门的四个主要用户和六个次要用户将从该仪器的可用性中受益。广泛的蛋白质被指定用于研究，包括血液蛋白质及其受体、参与信号传导和肌动蛋白组织的细胞内蛋白质、细胞外基质的蛋白质以及源自乳铁蛋白的防御素样抗菌肽。拟议的研究将更好地了解这些蛋白质在其各自正常和异常生理学中的作用，可能导致开发从癌症到阿尔茨海默氏症的各种疾病的改进疗法。