GENETIC ANALYSIS OF POLARITY

极性的遗传分析

• 批准号: 2025078
• 负责人: Ruth M STEWARD
• 金额: $ 19.42万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2025078
• 关键词: Drosophilidae; biological signal transduction; cellular polarity; developmental genetics; early embryonic stage; gene induction /repression; histogenesis; nucleoproteins; phosphorylation; yeast two hybrid system

The formation of the Dorsal nuclear gradient in the early Drosophila embryo represents the last step in the maternally encoded pathway that establishes dorsal-ventral polarity. The nuclear gradient is formed when, in response to a ventral signal, the inactive cytoplasmic Dorsal protein is dissociated from its inhibitor, Cactus, and targeted to the nucleus. These events are conserved in the larval fat body, where the Drosophila immune response occurs, and also in vertebrates in the activation of Re1 proteins. Vertebrate Rel proteins function in the development of the liver, thymus, and in hematopoiesis, as well as in the immune response. In addition, Rel proteins control the expression of viruses such as HIV. Our project focuses on the Dorsal pathway in early Drosophila embryogenesis. We propose to identify additional genes that function in the pathway, in particular, genes whose products directly interact with Dorsal and Cactus. We also propose to investigate the importance of phosphorylation for nuclear targeting, gradient formation, and the function of the Dorsal protein. We will also study the function of the Rel pathway, and the function of the rel homolog Dif in the humoral and cellular immune response.

早期背核梯度的形成 果蝇胚胎代表了母性进化的最后一步 建立背腹极性的编码途径。的 核梯度的形成是，响应于腹侧信号， 无活性细胞质Dorsal蛋白从其 抑制剂，仙人掌，并针对细胞核。这些事件 保存在幼虫脂肪体中，果蝇在那里免疫 反应发生，也在脊椎动物中的Re1激活 proteins.脊椎动物Rel蛋白在发育中的作用 在肝脏、胸腺、造血和免疫系统中 反应此外，Rel蛋白控制以下蛋白的表达： 病毒，如HIV。我们的项目集中在背通道， 早期果蝇胚胎发生我们建议， 在该途径中起作用的基因，特别是， 产品直接与Dorsal和Cactus互动。我们亦建议 研究磷酸化对细胞核 靶向、梯度形成和背侧肌的功能 蛋白我们还将研究Rel通路的功能， rel同系物Dif在体液和细胞免疫中的功能 免疫反应