GENETIC ANALYSIS OF POLARITY

描述（改编自研究者摘要）：Rel途径是 最好的信号转导途径。在脊椎动物中， 免疫和炎症反应基因的活性以及 病毒基因在果蝇中，Rel通路在先天免疫中起作用， 反应和建立背腹（DV）极性在早期 胚胎Rel蛋白还与肿瘤的发生和细胞凋亡有关， 脊椎动物 我们长期以来一直在研究Rel蛋白Dorsal的功能及其在细胞内的表达。 细胞质抑制剂，仙人掌。背部代表了最后一步 形态发生途径，在卵子发生早期启动的途径。它 建立一个在早期胚胎中转导的腹侧信号， 在背腹到背核梯度的形成中。一旦进入 核，背调控特定合子基因的表达， DV轴的建立以及将该轴细分为离散的 域. 我们的实验旨在阐明背核梯度是如何 以及背部功能是如何控制的。在此期间，我们 已经发现了四个新的基因，它们在上游发挥作用，或者与之协同作用， 仙人掌和背部。我们建议继续对其进行定性， 识别在该途径中起作用的其他基因。因为Rel途径 在苍蝇和人类中高度保守，很可能被鉴定的基因 在脊椎动物中也会发挥作用。

DESCRIPTION (Adapted from investigator's abstract): The Rel pathway is one of the best understood signal transduction pathways. In vertebrates, it controls the activity of the immune and inflammatory response genes as well as that of viral genes. In Drosophila, the Rel pathway functions in the innate immune response and in the establishment of dorsal-ventral (DV) polarity in the early embryo. Rel proteins are also implicated in oncogenesis and apoptosis in vertebrates. We have long been studying the function of the Rel protein Dorsal and its cytoplasmic inhibitor, Cactus. Dorsal represents the last step in a morphogenetic pathway, a pathway initiated during early oogenesis. It establishes a ventral signal that is transduced in the early embryo and results in the formation of the Dorsal ventral-to-dorsal nuclear gradient. Once in the nucleus, Dorsal regulates the expression of specific Zygotic genes resulting in the establishment of the DV axis and the subdivision of this axis into discreet domains. Our experiments are aimed at elucidating how the dorsal nuclear gradient is formed and how dorsal function is controlled. During this funding period we have identified four new genes functioning upstream from or in consort with cactus and dorsal. We propose to continue with their characterization and to identify additional genes functioning in the pathway. Because the Rel pathway is highly conserved in flies and humans, it is likely that the genes identified in our work will also function in vertebrates.