课题基金 / 基金详情

ULTRASONIC ENHANCEMENT OF ANTIBIOTIC ACTION ON BIOFILMS

ULTRASONIC ENHANCEMENT OF ANTIBIOTIC ACTION ON BIOFILMS
超声波增强抗生素对生物膜的作用
批准号:
2445272
负责人:
WILLIAM G PITT
金额:
$17.86万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-07-01 至 1999-06-30

项目摘要

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中文摘要
翻译
植入的医疗设备有时会受到感染,导致患者死亡
英文摘要
Implanted medical devices sometimes become infected, resulting in patient suffering and additional surgery for removal or replacement of the device. Implant related infections can usually be controlled with antibiotic therapy, but the infection can rarely be completely eliminated. Once the antibiotic therapy is stopped, the infection returns. The infectious organisms usually exude a polysaccharide slime that appears to protect the bacteria from antibiotic therapy and from the body's defense mechanisms. There are several hypotheses concerning the recalcitrance of the infection toward antibiotic therapy, most of which implicate the exopolysaccharide in reducing the transport of antibiotics or nutrients to the bacteria. The proposed research is centered on eliminating bacterial infections on implant devices using high frequency pressure waves (ultrasound) to work synergistically with antibiotics to kill bacterial biofilms on implant devices. Our preliminary results indicate that ultrasound can enhance transport of antibiotic to cells within the biofilm, and can enhance the action of antibiotics against both sessile and planktonic bacteria. For example, preliminary results show simultaneous application of gentamicin and 2.25 MHz ultrasound to P. aeruginosa biofilms reduces the number of viable cells in the biofilm by 3 orders of magnitude, and that 67 kHz ultrasound reduces viability of sessile and planktonic P. aeruginosa by nearly 3 orders of magnitude. This same level of ultrasonic stimulation has no effect on viability when no antibiotic is present. The effect is also observed with E. coil, and S. fecaelis, The specific aims of this proposal are designed to expand this synergistic effect of ultrasound and antibiotics to increase the killing of biofilms on solid surfaces, and to investigate the underlying physical and chemical mechanisms. 1.1 The project will expose a bacterial biofilm to antibiotics and ultrasound to determine if bacteria are killed beyond that accomplished by the antibiotics alone. Biofilms of P. aeruginosa, S. fecaelis, and E. coil will be tested on polyethylene, polyurethane, silicone rubber, and glass surfaces. 1.2 We have already demonstrated synergistic effects in killing P. aeruginosa and E. coli with gentamicin in combination with 2.25 MHz and 67 kHz ultrasound. We will try to optimize the killing by exploring other frequencies up to 10 MHz, various waveforms, power densities, and the use of another antibiotic. 1.3 We will identify the extent to which ultrasound enhances transport of antibiotic into a bacterium by using ESR spin-labeled antibiotics to measure the kinetics of uptake into bacteria with and without ultrasonic stimulation. This will demonstrate whether the membrane transport is perturbed by ultrasound. 1.4 We will identify the extent to which ultrasound enhances transport within a bacterial biofilm by measuring ultrasonic effects upon transport of antibiotics, oxygen and other molecules through the biofilm.
期刊论文(7)
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会议论文
Controlled Drug Delivery to Colon Tumors Via Ultrasound
  • 批准号:
    7057214
  • 项目类别:
  • 资助金额:
    $23.07万
  • 财政年份:
    2004
  • 负责人:
    WILLIAM G PITT
  • 依托单位:
Controlled Drug Delivery to Colon Tumors Via Ultrasound
  • 批准号:
    6890959
  • 项目类别:
  • 资助金额:
    $23.63万
  • 财政年份:
    2004
  • 负责人:
    WILLIAM G PITT
  • 依托单位:
Controlled Drug Delivery to Colon Tumors Via Ultrasound
  • 批准号:
    6780136
  • 项目类别:
  • 资助金额:
    $24.6万
  • 财政年份:
    2004
  • 负责人:
    WILLIAM G PITT
  • 依托单位:
ULTRASONIC/ANTIBIOTIC THERAPY--INFECTED IMPLANT IN VIVO
  • 批准号:
    2563425
  • 项目类别:
  • 资助金额:
    $12.52万
  • 财政年份:
    1998
  • 负责人:
    WILLIAM G PITT
  • 依托单位:
国内基金
海外基金
生物质炭负载噬菌体对土壤中抗生素耐药菌(Pseudomonas aeruginosa)迁移阻控及靶向裂解的协同机制
  • 批准号:
    42077106
  • 项目类别:
    面上项目
  • 资助金额:
    57.0万元
  • 批准年份:
    2020
  • 负责人:
    孙明明
  • 依托单位:
融合自组装双亲短肽提高Pseudomonas aeruginosa脂肪氧合酶热稳定性机制的研究
  • 批准号:
    31401638
  • 项目类别:
    青年科学基金项目
  • 资助金额:
    22.0万元
  • 批准年份:
    2014
  • 负责人:
    刘松
  • 依托单位:
铜绿假单胞菌(Pseudomonas aeruginosa)SU8抑菌活性物质吩嗪-1-甲酰胺结构改造及增效作用研究
  • 批准号:
    31301709
  • 项目类别:
    青年科学基金项目
  • 资助金额:
    23.0万元
  • 批准年份:
    2013
  • 负责人:
    张亚
  • 依托单位:
铜绿假单胞菌(Pseudomonas aeruginosa)作用下PBS及其共聚物的降解途径研究
  • 批准号:
    21144008
  • 项目类别:
    专项基金项目
  • 资助金额:
    10.0万元
  • 批准年份:
    2011
  • 负责人:
    张敏
  • 依托单位: