CORE--CELLULAR MORPHOLOGY

细胞形态学核心实验室的建设是为了满足 细胞生物学和转基因检测领域的需求不断增加 基因治疗研究。 冷冻形式的常规形态支持， 用于光水平显微镜 (LM) 的石蜡和甲基丙烯酸乙二醇酯切片 以及标准透射电子显微镜（EM） 作为为参与 P01 项目的研究人员提供的综合服务。 此外，核心将强调最先进的方法 检测转基因表达，包括原位杂交， β-半乳糖苷酶的免疫细胞化学和组织化学检测 碱性磷酸酶报告基因。 细胞形态核心将 提供原位杂交和免疫细胞化学技术 转基因检测作为临床前研究的综合服务 动物模型核心。 细胞形态核心将与 通过两种机制参与P01项目； i) 全面的服务， ii) 设施和技术支持。 综合服务将 主要应用临床前项目。 这将确保最小 大量的技术可变性和高质量保证。 或者， CMC将提供原位杂交综合服务， 免疫细胞化学和组织化学贯穿最初的发育 与 PO1 项目相关的研究阶段。 一旦方法为 发展研究已在核心、技术中得到定义 将移交给个别调查员。 在这个阶段 开发项目，用户将完全支持常规切片 并完全获得专业设备和常用试剂 技术。 由于电子显微镜的特殊性，所有 EM 服务将作为综合服务提供。 约翰·恩格尔哈特 (John Engelhardt) 博士作为细胞形态学核心的指导老师 将是该 DMD 形态学核心组件的首席研究员。 他发表了涉及 CFTR 本地化的研究 人支气管中的杂交和免疫细胞化学以及一些 使用基因转移技术来更好地理解的手稿 与重组有关的宿主-载体相互作用的生物学 异种移植物、小鼠近端呼吸道上皮中的腺病毒 肝、棉鼠肺。

The Cellular morphology Core laboratory is constructed to meet the increasing demands of cell biology and transgene detection in the area of gene therapy research. Routine morphology support in the form of frozen, paraffin, and glycolmethacrylate sectioning for light level microscopy (LM) as well as standard transmission electron microscopy (EM) will be provided as a comprehensive service to investigators participating in P01 projects. In addition, the core will emphasize state of the art methodologies for detecting transgene expression including in situ hybridization, immunocytochemistry,a nd histochemical detection of beta-galactosidase and alkaline phosphatase reporter genes. The cellular morphology core will provide techniques of in situ hybridization and immunocytochemical detection of transgenes as comprehensive services to preclinical studies of the Animal Models Core. The cellular morphology core will interact with participating P01 projects by two mechanisms; i) comprehensive service, and ii) facilities and technical support. Comprehensive services will be predominantly applied preclinical projects. This will assure athe minimal amount of technical variability and high quality assurance. Alternatively, the CMC will provide comprehensive services of in situ hybridization, immunocytochemistry, and histochemistry through the initial developmental stages of studies pertaining to PO1 projects. Once methods for developmental studies have been defined within the core, the technologies will be transferred to individual investigators. During this phase of developmental projects, users will have full support for routine sectioning and full access to equipment and commonly used reagents for specialized techniques. Due to the specialized nature of electron microscopy, all EM services will be provided as a comprehensive service. As Faculty Advisor tot he Cellular Morphology Core, Dr. John Engelhardt will be the principal investigator of this DMD Morphology Core component. He has published studies involving CFTR localization by in situ hybridization and immunocytochemistry in human bronchus as well as several manuscripts which used gene transfer technology to better understand the biology of host-vector interactions as it pertains to recombinant adenovirus in human proximal respiratory epithelium in xenografts, mouse liver, and cotton rat lung.