CORE--CELLULAR MORPHOLOGY

核心--细胞形态学

• 批准号: 6100646
• 负责人: JOHN ENGELHARDT
• 金额: --
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100646
• 关键词: biomedical facility; electron microscopy; genetic techniques; genetically modified animals; histochemistry /cytochemistry; immunocytochemistry; in situ hybridization; morphology; tissue /cell preparation

The Cellular morphology Core laboratory is constructed to meet the increasing demands of cell biology and transgene detection in the area of gene therapy research. Routine morphology support in the form of frozen, paraffin, and glycolmethacrylate sectioning for light level microscopy (LM) as well as standard transmission electron microscopy (EM) will be provided as a comprehensive service to investigators participating in P01 projects. In addition, the core will emphasize state of the art methodologies for detecting transgene expression including in situ hybridization, immunocytochemistry,a nd histochemical detection of beta-galactosidase and alkaline phosphatase reporter genes. The cellular morphology core will provide techniques of in situ hybridization and immunocytochemical detection of transgenes as comprehensive services to preclinical studies of the Animal Models Core. The cellular morphology core will interact with participating P01 projects by two mechanisms; i) comprehensive service, and ii) facilities and technical support. Comprehensive services will be predominantly applied preclinical projects. This will assure athe minimal amount of technical variability and high quality assurance. Alternatively, the CMC will provide comprehensive services of in situ hybridization, immunocytochemistry, and histochemistry through the initial developmental stages of studies pertaining to PO1 projects. Once methods for developmental studies have been defined within the core, the technologies will be transferred to individual investigators. During this phase of developmental projects, users will have full support for routine sectioning and full access to equipment and commonly used reagents for specialized techniques. Due to the specialized nature of electron microscopy, all EM services will be provided as a comprehensive service. As Faculty Advisor tot he Cellular Morphology Core, Dr. John Engelhardt will be the principal investigator of this DMD Morphology Core component. He has published studies involving CFTR localization by in situ hybridization and immunocytochemistry in human bronchus as well as several manuscripts which used gene transfer technology to better understand the biology of host-vector interactions as it pertains to recombinant adenovirus in human proximal respiratory epithelium in xenografts, mouse liver, and cotton rat lung.

细胞形态学核心实验室的建设，以满足 细胞生物学和转基因检测领域的需求不断增加， 基因治疗研究。 常规形态学支持， 石蜡和乙二醇甲基丙烯酸酯切片，用于光学显微镜（LM） 以及标准的透射电子显微镜（EM）将提供 为参与P01项目的研究者提供全面的服务。 此外，核心将强调最先进的方法， 检测转基因表达包括原位杂交， 免疫细胞化学和β-半乳糖苷酶组织化学检测， 碱性磷酸酶报告基因。 细胞形态学核心将 提供原位杂交和免疫细胞化学技术 转基因检测作为临床前研究的综合服务 动物模型核心 细胞形态核心将与 通过两种机制参与P01项目：i）综合服务， （ii）设施及技术支援。 全面的服务将 主要应用于临床前项目。 这将确保最小的 大量的技术可变性和高质量保证。 可选择地， CMC将提供原位杂交， 免疫细胞化学和组织化学通过最初的发育 与PO 1项目有关的研究阶段。 一旦方法用于 发展研究已被定义为核心技术， 将转交给个别调查人员。 在这一阶段， 发展项目，用户将得到全面支持， 和充分获得设备和常用试剂， 技术. 由于电子显微镜的专业性质，所有EM 服务将作为一项综合服务提供。 作为细胞形态学核心的教师顾问，John Engelhardt博士 将是DMD形态学核心组件的主要研究者。 他已经发表了涉及CFTR本地化的研究， 人支气管及几种支气管上皮细胞杂交和免疫细胞化学 使用基因转移技术来更好地理解 宿主-载体相互作用的生物学，因为它涉及重组 异种移植物中人近端呼吸道上皮中的腺病毒，小鼠 肝脏和棉鼠肺。