CORE--CELLULAR MORPHOLOGY

细胞形态核心实验室的建设是为了满足 对细胞生物学和转基因检测领域日益增长的需求 基因治疗研究。常规形态支持以冰冻的形式， 石蜡切片和甲基丙烯酸乙酯切片用于光水平显微镜(LM) 以及标准的透射电子显微镜(EM) 作为对参与P01项目的调查人员的全面服务。 此外，核心将强调最新的方法论 检测包括原位杂交在内的转基因表达， 免疫细胞化学、β-半乳糖苷酶和组织化学检测 碱性磷酸酶报告基因。细胞形态核心将 提供原位杂交和免疫细胞化学技术 转基因检测作为临床前研究的综合服务 动物模型核心。细胞形态核心将与 通过两个机制参与P01项目：一)综合服务， 和二)设施和技术支持。全面的服务将是 主要应用于临床前项目。这将保证最低限度的 技术变化量大，质量保证高。或者， CMC将提供全面的原位杂交服务， 免疫细胞化学和组织化学在发育初期 与“小一”项目有关的研究阶段。一次方法用于 发展研究已经被定义在核心技术中 将被移交给个人调查人员。在这一阶段， 开发项目，用户将获得对常规切片的全面支持 并完全可以获得设备和常用试剂，用于专业 技巧。由于电子显微镜的专业性，所有的EM 服务将作为一项全面的服务提供。 约翰·恩格尔哈特博士担任细胞形态核心教研室顾问 将是这一DMD形态核心组件的首席研究员。 他发表了关于CFTR原位定位的研究 人支气管及几种支气管壁的杂交和免疫细胞化学 利用基因转移技术更好地理解 宿主-载体相互作用的生物学，因为它属于重组 人近端呼吸道上皮腺病毒在异种移植中的表达 肝脏和棉鼠肺。

The Cellular morphology Core laboratory is constructed to meet the increasing demands of cell biology and transgene detection in the area of gene therapy research. Routine morphology support in the form of frozen, paraffin, and glycolmethacrylate sectioning for light level microscopy (LM) as well as standard transmission electron microscopy (EM) will be provided as a comprehensive service to investigators participating in P01 projects. In addition, the core will emphasize state of the art methodologies for detecting transgene expression including in situ hybridization, immunocytochemistry,a nd histochemical detection of beta-galactosidase and alkaline phosphatase reporter genes. The cellular morphology core will provide techniques of in situ hybridization and immunocytochemical detection of transgenes as comprehensive services to preclinical studies of the Animal Models Core. The cellular morphology core will interact with participating P01 projects by two mechanisms; i) comprehensive service, and ii) facilities and technical support. Comprehensive services will be predominantly applied preclinical projects. This will assure athe minimal amount of technical variability and high quality assurance. Alternatively, the CMC will provide comprehensive services of in situ hybridization, immunocytochemistry, and histochemistry through the initial developmental stages of studies pertaining to PO1 projects. Once methods for developmental studies have been defined within the core, the technologies will be transferred to individual investigators. During this phase of developmental projects, users will have full support for routine sectioning and full access to equipment and commonly used reagents for specialized techniques. Due to the specialized nature of electron microscopy, all EM services will be provided as a comprehensive service. As Faculty Advisor tot he Cellular Morphology Core, Dr. John Engelhardt will be the principal investigator of this DMD Morphology Core component. He has published studies involving CFTR localization by in situ hybridization and immunocytochemistry in human bronchus as well as several manuscripts which used gene transfer technology to better understand the biology of host-vector interactions as it pertains to recombinant adenovirus in human proximal respiratory epithelium in xenografts, mouse liver, and cotton rat lung.