IDENTIFICATION AND CHARACTERIZATION OF A NOVAL PEPTIDE OSTEOCLASTPOIETIC FACTOR
IDENTIFICATION AND CHARACTERIZATION OF A NOVAL PEPTIDE OSTEOCLASTPOIETIC FACTOR
新型肽破骨细胞生成因子的鉴定和表征
批准号：
2336156
负责人：
金额：
$0.0万
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
关键词：
animal tissue autoradiography bioassay bone metabolism disorder chickens cytokine gene expression genetic library human subject immunoaffinity chromatography laboratory mouse laboratory rat molecular cloning organ culture osteoclast activating factor osteoclasts osteogenesis pathologic bone resorption physiologic bone resorption polymerase chain reaction protein purification protein sequence
中文摘要
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英文摘要
We have identified a novel factor which stimulates the formation of
osteoclasts and which is produced by a human tumor as well as by
leukocytes which have been stimulated with concanavalin A. We have called
this peptide osteoclastpoietic factor (OPF). We have obtained amino acid
sequence on this peptide and found that it is novel and distinct from
other known growth regulatory factors, interleukins, and colony
stimulating factors. We have found that a synthetic peptide which
corresponds to this amino acid sequence mimics the biological effects,
and we have produced both monoclonal and polyclonal antibodies which
neutralize the original biological activity. In this project, we plan
to:
1. Clone the gene and express the recombinant molecule.
2. Determine the role of OPF in normal bone remodeling and in disease
states associated with increased bone resorption.
Characterization of novel cytokines which are powerful stimulators of
osteoclastic bone resorption is likely to lead to new insights in the
normal control of osteoclast function and in the pathophysiology of
disease states associated with bone destruction.
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