GROWTH HORMONE SIGNALING TO THE NUCLEUS

生长激素向细胞核发出信号

• 批准号: 2444080
• 负责人: Jessica Schwartz
• 金额: $ 15.92万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2444080
• 关键词: CHO cells; DNA binding protein; DNA methylation; cell nucleus; cytokine; genetic promoter element; genetic transcription; hormone regulation /control mechanism; intermolecular interaction; nucleic acid probes; nucleic acid sequence; phosphorylation; protooncogene; somatotropin; tissue /cell culture; transfection

DESCRIPTION (Adapted from the applicant's abstract): The applicant proposes to investigate the hypothesis that multiple GH-regulated signaling pathways interact at various levels into networks which lead to specific responses to GH. These interactions occur at the level of transcription factors and their binding, as well as by crosstalk among more proximal signaling molecular between the GH receptor and the nucleus. To examine this hypothesis the applicant plans to analyze transcriptional regulation of the c-fos gene by GH. Four Specific Aims are proposed: (1) To define interactions among GH-regulated DNA-protein complexes associated with the c-fos gene; (2) To identify interactions among GH-responsive DNA sequences by analysis of DNA-binding proteins; (3) To examine regulation by GH of DNA-binding complexes; (4) To determine how interactions in DNA-protein complexes contribute to specificity in GH versus cytokine regulation of c-fos transcription.

描述（改编自申请人摘要）：申请人提出