GROWTH HORMONE SIGNALING TO THE NUCLEUS

描述（改编自申请人摘要）：申请人提出 研究多种生长激素调节的信号通路 在各级相互作用，形成网络， GH。 这些相互作用发生在转录因子水平， 它们的结合，以及更近端的信号传导之间的串扰， GH受体和细胞核之间的分子。 审查这个 假设申请人计划分析基因的转录调节 c-fos基因的表达。 提出了四个具体目标：（1）定义 生长激素调节的DNA-蛋白质复合物之间的相互作用 c-fos基因;（2）鉴定GH应答DNA序列之间的相互作用 通过分析DNA结合蛋白;（3）检查GH对 DNA结合复合物;（4）确定DNA-蛋白质相互作用 复合物有助于GH对细胞因子调节的特异性， c-fos转录。

DESCRIPTION (Adapted from the applicant's abstract): The applicant proposes to investigate the hypothesis that multiple GH-regulated signaling pathways interact at various levels into networks which lead to specific responses to GH. These interactions occur at the level of transcription factors and their binding, as well as by crosstalk among more proximal signaling molecular between the GH receptor and the nucleus. To examine this hypothesis the applicant plans to analyze transcriptional regulation of the c-fos gene by GH. Four Specific Aims are proposed: (1) To define interactions among GH-regulated DNA-protein complexes associated with the c-fos gene; (2) To identify interactions among GH-responsive DNA sequences by analysis of DNA-binding proteins; (3) To examine regulation by GH of DNA-binding complexes; (4) To determine how interactions in DNA-protein complexes contribute to specificity in GH versus cytokine regulation of c-fos transcription.