GROWTH HORMONE SIGNALING TO THE NUCLEUS

描述(改编自申请人的摘要)：申请人提出 研究多条生长激素调节的信号通路的假说 在不同层面上相互作用，形成网络，从而对 天哪。这些相互作用发生在转录因子和 它们的结合，以及更接近的信号之间的串扰 位于生长激素受体和细胞核之间的分子。要检查这一点 假设申请者计划分析转录调控 生长激素诱导的c-fos基因。提出了四个具体目标：(1)明确 生长激素调节的DNA-蛋白质复合体之间的相互作用 C-fos基因；(2)鉴定生长激素反应DNA序列之间的相互作用 通过DNA结合蛋白的分析；(3)检测生长激素对 DNA结合复合体；(4)确定DNA-蛋白质之间的相互作用 复合体在生长激素与细胞因子调节中的特异性 C-fos转录。

DESCRIPTION (Adapted from the applicant's abstract): The applicant proposes to investigate the hypothesis that multiple GH-regulated signaling pathways interact at various levels into networks which lead to specific responses to GH. These interactions occur at the level of transcription factors and their binding, as well as by crosstalk among more proximal signaling molecular between the GH receptor and the nucleus. To examine this hypothesis the applicant plans to analyze transcriptional regulation of the c-fos gene by GH. Four Specific Aims are proposed: (1) To define interactions among GH-regulated DNA-protein complexes associated with the c-fos gene; (2) To identify interactions among GH-responsive DNA sequences by analysis of DNA-binding proteins; (3) To examine regulation by GH of DNA-binding complexes; (4) To determine how interactions in DNA-protein complexes contribute to specificity in GH versus cytokine regulation of c-fos transcription.