REGULATION OF GENE EXPRESSION BY GROWTH HORMONE & RELATED GROWTH FACTORS

生长激素对基因表达的调节

• 批准号: 6263646
• 负责人: Jessica Schwartz
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6263646
• 关键词: 3T3 cells; antisense nucleic acid; biological signal transduction; gene expression; genetic regulatory element; genetic transcription; hormone receptor; hormone regulation /control mechanism; protein tyrosine kinase; protooncogene; somatotropin; transcription factor

Regulation of gene expression by GH is a focus to understand the mechanism of action of GH. In 3T3-F442A cells, GH induces c-fos transcription, via the serum response element (SRE). We are investigating involvement of transcription factors associated with the SRE and with the SIF-binding sequence in GH action. Other approaches include studying signaling between GH receptors and c-fos tyrosine kinase, and assessment of GH action in cells expressing anti-sense c-fos.

GH对基因表达的调控是了解GH作用机制的重点。 在 3T3-F442A 细胞中，GH 通过血清反应元件 (SRE) 诱导 c-fos 转录。 我们正在研究与 SRE 和 SIF 结合序列相关的转录因子在 GH 作用中的参与。 其他方法包括研究 GH 受体和 c-fos 酪氨酸激酶之间的信号传导，以及评估表达反义 c-fos 的细胞中的 GH 作用。