NF2 BINDING PROTEINS

该项目是跟进先前的酵母两个杂交研究， 鉴定了与NF 2蛋白相互作用的蛋白质。 PI打算 免疫共沉淀将探索蛋白质中涉及的结构域 使用截短的NF2构建体的相互作用，并将克隆和鉴定 编码相互作用蛋白的全长基因。 于是他又会 制备针对这些NF2结合蛋白的抗体，以探索 他们的定位。 将如何利用永生化雪旺细胞来探索 反义结合蛋白的功能属性。 他将 研究各种自然发生的NF2突变对 交互. 最后，他将确定 FISH检测结合蛋白。

The project is to follow up on prior yeast two hybrid studies that identified proteins that interact with the NF2 protein. The PI intends co-immunoprecipitations will explore the domains involved in the protein interactions using truncated NF2 constructs and will clone and identify the full length genes encoding the interacting proteins. Then, he will prepare antibodies against these NF2 binding proteins in order to explore their localizations. How will use immortalized Schwann cells to explore the functional attributes of the binding proteins by antisense. He will examine the effects of various naturally occurring NF2 mutations on the interactions. Finally, he will determine the chromosomal localizations of the binding proteins by FISH.