NF2 BINDING PROTEINS

NF2结合蛋白

• 批准号: 2796990
• 负责人: Daniel R Scoles
• 金额: $ 3.28万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-22 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2796990
• 关键词: Schwann cells; antisense nucleic acid; binding proteins; fluorescent in situ hybridization; gene mutation; immunoprecipitation; molecular cloning; neurotrophic factors; protein structure function

The project is to follow up on prior yeast two hybrid studies that identified proteins that interact with the NF2 protein. The PI intends co-immunoprecipitations will explore the domains involved in the protein interactions using truncated NF2 constructs and will clone and identify the full length genes encoding the interacting proteins. Then, he will prepare antibodies against these NF2 binding proteins in order to explore their localizations. How will use immortalized Schwann cells to explore the functional attributes of the binding proteins by antisense. He will examine the effects of various naturally occurring NF2 mutations on the interactions. Finally, he will determine the chromosomal localizations of the binding proteins by FISH.

该项目是对之前的酵母两个杂交研究的后续研究 确定了与NF2蛋白相互作用的蛋白质。私家侦探打算 免疫共沉淀法将探索蛋白质中涉及的区域。 使用截断的NF2结构的相互作用，并将克隆和识别 编码相互作用蛋白的全长基因。那么，他就会 制备针对这些NF2结合蛋白的抗体以探索 他们的地方化。将如何利用永生化的雪旺细胞来探索 反义结合蛋白的功能属性。他会的 检查各种自然发生的NF2突变对 互动。最后，他将确定人类的染色体定位。 鱼的结合蛋白。