AXON MIGRATION AND TERMINATION IN C ELEGANS

线虫中的轴突迁移和终止

• 批准号: 2420750
• 负责人: Susan Kirch
• 金额: $ 2.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1997-12-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2420750
• 关键词: Caenorhabditis elegans; afferent nerve; animal genetic material tag; axon; complementary DNA; cyclic GMP; developmental genetics; developmental neurobiology; enzyme activity; guanylate cyclase; molecular cloning; neural transmission; neurogenetics; neuronal guidance; nucleic acid sequence; synaptogenesis

How are precise patterns of synaptic connections formed during the development of a nervous system? Establishment of these patterns require both neuronal activity-dependent and activity-independent mechanisms. It is clear that axons stop growing in response to signals from their targets (and sometimes other cells), but it is not clear what those signals are or how they are generated. C.elegans is a model organism in which to study this phenomenon because of its powerful genetics and extremely well characterized nervous system. Recently, three genes were cloned which specifically affect the axon migration and termination of one chemosensory neuron, ASJ. The identity of these genes led to the proposal that cGMP signalling plays an important role in the proper guidance and termination of this axon. The first aim of this proposal is to determine what role electrical activity, synaptic transmission, , and the targets of ASJ have on the migration and termination of its axon, as well as to investigate the functional importance of cGMP signalling in axon migration. The second aim of this proposal is to isolate and characterize novel genes required for ASJ guidance through a visual genetic screen.

如何精确的突触连接模式在 神经系统的发展？建立这些模式需要 神经元活性依赖性和与活动无关的机制。它 很明显，轴突因其目标的信号而停止生长 （有时还有其他单元格），但尚不清楚这些信号是什么或 它们的生成方式。 C.Elegans是一种研究的模型生物 这种现象是因为其强大的遗传学和非常好 神经系统的特征。最近，克隆了三个基因 特别影响一种化学感应的轴突迁移和终止 神经元，ASJ。这些基因的身份导致了CGMP的提议 信号在适当的指导和终止中起重要作用 这个轴突。该提议的第一个目的是确定角色 电活动，突触传递，ASJ的目标 关于其轴突的迁移和终止以及调查 CGMP信号在轴突迁移中的功能重要性。第二个 该建议的目的是隔离和表征所需的新基因 通过视觉遗传筛选为ASJ引导。