MECHANISMS OF SENSORY AXON GUIDANCE

感觉轴突引导机制

• 批准号: 6070602
• 负责人: Susan Kirch
• 金额: $ 3.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6070602
• 关键词: Caenorhabditis elegans; gene mutation; genetic regulation; genotype; molecular cloning; neurogenetics; neuronal guidance; phenotype; sensory mechanism

Worms depend on taste, touch and smell to sense and explore their environment. Appropriate responses to information received through these different modalities depends on precise connectivity between sensory neurons and downstream effector neurons in the nerve ring. Little is known about how extending axons migrate through this nerve ring neuropil during development to find their partners. How is positional information interpreted by an extending axon so that desired synaptic partners are found? Focusing on the guidance of the ASI chemosensory neurons to their appropriate positions in the nerve ring, a mutant screen led to the isolation of 16 candidate mutant strains that appeared to have ASI axon guidance and termination phenotypes. These mutations define at least five new genes named sax-10-14 (sensory axon guidance). Four main phenotypic classes of ASI axon defects have been observed and the morphologies of other neurons are being determined. To facilitate genetic analysis and cloning of the sax genes, the genes have been mapped and attempts to rescue the sax-10 phenotype by cosmid injections and germline transformation are ongoing. Finally, analysis of known mutants for effects on axon guidance in the nerve ring will provide insight on what mechanisms are required for axon guidance and nerve ring development.

蠕虫取决于口味，触摸和气味来感知和探索其环境。 对通过这些不同方式收到的信息的适当响应取决于神经环中感觉神经元和下游效应神经元之间的精确连通性。 关于在发育过程中延伸轴突如何通过这种神经环神经迁移以找到其伴侣的方式知之甚少。如何通过扩展的轴突解释位置信息，从而找到所需的突触伙伴？ 关注ASI化学感应神经元在神经环中适当位置的引导，突变筛选导致了16个候选突变菌株的隔离，这些突变菌株似乎具有ASI轴突指导和终止表型。 这些突变定义了至少五个名为SAX-10-14的新基因（感觉轴突引导）。已经观察到了四个主要的ASI轴突缺陷的主要表型类，并且正在确定其他神经元的形态。 为了促进萨克斯基因的遗传分析和克隆，已经映射了这些基因，并试图通过宇宙注射和种系转化来挽救SAX-10表型。 最后，分析已知突变体对神经环中轴突引导的影响的分析将洞悉轴突引导和神经环发育所需的机制。