NEUROMODULATION OF HEART INTERNEURONS IN THE LEECH

水蛭中心脏中间神经元的神经调节

• 批准号: 2519899
• 负责人: Andrew A Hill
• 金额: $ 2.44万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2519899
• 关键词: Hirudinea; action potentials; alternatives to animals in research; computational neuroscience; computer simulation; heart innervation; heart rhythm; interneurons; invertebrate hormone; neural conduction; neural information processing; neural inhibition; neural transmission; neuropeptides; psychomotor function; voltage /patch clamp; voltage gated channel

Oscillatory neural networks underlie rhythmic behaviors such as respiration, heartbeat, and swimming. Recent theoretical and experimental work on these networks in invertebrates has highlighted cellular mechanisms that may be critical to the origin of the oscillation. Experimental and neuromodulatory manipulations of these critical voltage- gated and synaptic conductances in the leech heartbeat network will determine their influence on the oscillation period and waveform. The relative influence of graded and spike-mediated synaptic inhibition on the cycle period will be studied experimentally by altering the maximal conductances of these two modes of inhibition. Also, the effect of varying the strength of the hyperpolarization-activated inward current, I-h, which is thought to pace the heartbeat cycle, will be tested experimentally. In addition, a search will be made for a neuromodulator of Ih. These experiments will be done in conjunction with computer simulations of the network. The previously described effects of FMRFamide modulation on the delayed rectifier, Ik1, and spike-mediated transmission will be modeled. By determining the critical cellular mechanisms involved in rhythm generation in the leech, this study may reveal potential cellular targets of neuromodulation in motor-pattern generating networks in general.

振荡神经网络是节律行为的基础， 呼吸心跳和游泳最近的理论和实验 在无脊椎动物中对这些网络的研究突出了细胞网络 可能对振荡的起源至关重要的机制。 实验和神经调节操纵这些临界电压- 水蛭心跳网络中的门控和突触电导 确定它们对振荡周期和波形的影响。的 分级和棘波介导的突触抑制的相对影响 将通过改变最大的 这两种抑制模式的电导。另外， 改变超极化激活的内向电流的强度， I-h被认为是心跳周期的起搏器，将接受测试 实验性的此外，还将寻找一种神经调节剂， 的Ih。这些实验将结合计算机进行 网络的模拟。先前描述的FMRFamide的作用 对延迟整流、Ik 1和棘波介导的传递的调制 将被模仿。通过确定关键的细胞机制 这项研究可能揭示了水蛭节律产生的潜力 运动模式生成网络中神经调节的细胞靶点 梗概.