CASANOVA, A GENE CONTROLLING SEX-SPECIFIC BEHAVIOR

Casanova，一种控制性别特异性行为的基因

• 批准号: 2379554
• 负责人: KIM D. FINLEY
• 金额: $ 2.99万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-16 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2379554
• 关键词: CASANOVA; GENE; CONTROLLING; SEX; SPECIFIC

The goal of this research proposal is the molecular isolation and characterization of a novel gene named Casanova (cas),which is involved with the regulation of sex-specific behavior in Drosophila. Further characterization of the cas phenotype will determine the type behavioral abnormalities involved. P-element mutagenesis will be used to create deletions removing the cas gene or new alleles. New deletions will be used to further map the position of the cas gene to the smallest possible region. Characterized phage and cosmid clones, located within the region will be ordered and placed relative to their position on the deletion lines. Restriction fragments which identify transcription units and are placed within the smallest cas containing region will be used for the isolation of cDNA clones.

这项研究计划的目标是分子分离和 一个新基因Casanova(Cas)的特征 对果蝇的性别行为进行了调控。进一步 CAS表型的特征将决定类型行为 涉及到异常情况。将使用P-元素突变来创建 去除cas基因或新等位基因的缺失。新的删除内容将是 用于将cas基因的位置进一步定位到尽可能小的位置。 区域。位于该区域内的特征化噬菌体和粘粒克隆 将相对于它们在删除时的位置进行排序和放置 台词。识别转录单位的限制性片段 放置在包含CA的最小区域中，将用于 CDNA克隆的分离。