ANALYSES OF SITE SPECIFIC RECOMBINATION
ANALYSES OF SITE SPECIFIC RECOMBINATION
批准号:
2021859
负责人:
JEFFREY F GARDNER
金额:
$26.54万
依托单位国家:
美国
项目类别:
财政年份:
1980
资助国家:
美国
项目状态:
已结题
起止时间:
1980-12-01 至 2000-12-31
中文摘要
位点特异性重组系统在控制
发展和基因表达在各种各样的生物体,
从细菌到人类。该项目的长期目标是
了解噬菌体X如何进行位点特异性重组。的
调查结果将对其他特定地点产生重大影响
重组系统,因为它们的许多整合酶与重组系统相关。
λ Int蛋白。生物化学和遗传学方法将用于
表征蛋白质-蛋白质和蛋白质-DNA相互作用,
在重组复合物(整合体)的组装过程中,
链断裂和交换的过程。噬菌体编码整合酶(Int)
蛋白质是整合体形成和链交换的中心作用物。Int
突变体将被分离并在测定中表征,
重组途径中单个蛋白质的缺陷。的
宿主编码的整合宿主因子(IHF)也参与整合体的形成。
通过诱导DNA弯曲来形成。我们会隔离那些
如我们先前的研究所建议的,
基因研究,与DNA相互作用。这些突变体还将提供
这些信息将有助于解释来自物理
研究蛋白质。噬菌体编码的切除酶(Xis)和宿主-
反转刺激编码因子(FIS)促进切除
重组Xis与Int和FIS协同作用。我们将
表征蛋白质-蛋白质相互作用的协同机制
通过分离和表征Xis突变体,
与Int或FIS交互。
英文摘要
Site-specific recombination systems are important in controlling
development and gene expression in a diverse array of organisms ranging
from bacteria to humans. The long range goal of this project is to
understand how bacteriophage X carries-out site-specific recombination. The
findings will have a significant impact on other site-specific
recombination systems because many of their integrases are related to the
lambda Int protein. Biochemical and genetic approaches will be used to
characterize the protein-protein and protein-DNA interactions that occur
both during the assembly of recombination complexes (intasomes) and the
process of strand-cleavage and exchange. The phage-encoded integrase (Int)
protein is a central actor in intasome formation and strand exchange. Int
mutants will be isolated and characterized in assays that will determine
the defects of the individual proteins in the recombination pathway. The
host-encoded integration host factor (IHF) also participates in intasome
formation by inducing bends in the DNA. We will isolate mutants that have
amino acid substitutions of residues that, as suggested by our previous
genetic studies, interact with DNA. Such mutants will also provide
information that will be useful in interpreting data derived from physical
studies on the protein. The phage-encoded excisionase (Xis) and the host-
encoded factor for inversion stimulation (FIS) promote excisive
recombination. Xis interacts cooperatively with Int and FIS. We will
characterize the mechanism(s) of cooperative protein-protein interactions
by isolating and characterizing Xis mutants that are defective in
interacting with Int or FIS.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Conjugal Transfer of Bacteriodes Antibiotic Resistances
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批准号:8321682
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项目类别:
-
资助金额:$35.93万
-
财政年份:1985
-
负责人:JEFFREY F GARDNER
-
依托单位:
ANALYSES OF SITE SPECIFIC RECOMBINATION
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批准号:6138379
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项目类别:
-
资助金额:$28.97万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
SITE-SPECIFIC RECOMBINATION
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批准号:2175257
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项目类别:
-
资助金额:$24.37万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:7102410
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项目类别:
-
资助金额:$29.42万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
ANALYSES OF SITE SPECIFIC RECOMBINATION
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批准号:2857090
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项目类别:
-
资助金额:$28.13万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
REGULATORY MUTATIONS IN THE THREONINE OPERON
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批准号:3276000
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项目类别:
-
资助金额:$17.39万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
REGULATORY MUTATIONS IN THE THREONINE OPERON
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批准号:3275997
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项目类别:
-
资助金额:$13.13万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:7078892
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项目类别:
-
资助金额:$9.79万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
SITE-SPECIFIC RECOMBINATION
-
批准号:2175258
-
项目类别:
-
资助金额:$26.46万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
ANALYSES OF SITE SPECIFIC RECOMBINATION
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批准号:2634635
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项目类别:
-
资助金额:$27.32万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
ANALYSES OF SITE-SPECIFIC RECOMBINATION
-
批准号:3275995
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项目类别:
-
资助金额:$20.81万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:7193481
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项目类别:
-
资助金额:$30.04万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:8496804
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项目类别:
-
资助金额:$32.16万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:8296552
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项目类别:
-
资助金额:$33.32万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
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批准号:6758550
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项目类别:
-
资助金额:$27.67万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
REGULATORY MUTATIONS IN THE THREONINE OPERON
-
批准号:3275996
-
项目类别:
-
资助金额:$11.96万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
Analyses of Site-Specific Recombination
-
批准号:8075440
-
项目类别:
-
资助金额:$33.32万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
REGULATORY MUTATIONS IN THE THREONINE OPERON
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批准号:3276002
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项目类别:
-
资助金额:$18.4万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
REGULATORY MUTATIONS IN THE THREONINE OPERON
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批准号:3275999
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项目类别:
-
资助金额:$16.8万
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财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
SITE-SPECIFIC RECOMBINATION
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批准号:2175256
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项目类别:
-
资助金额:$23.53万
-
财政年份:1980
-
负责人:JEFFREY F GARDNER
-
依托单位:
海外基金